Product Details
| Alternative Name: | HMOX1, Hsp32, Heat shock protein 32, Heme oxygenase 1 |
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| Clone: | HO-1-2 |
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| Host: | Mouse |
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| Isotype: | IgG2b |
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| Immunogen: | Native rat HO-1. |
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| UniProt ID: | P06762 |
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| GenBank ID: | J02722 |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Mouse, Rat
Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit
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| Applications: | Flow Cytometry, IHC, WB
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| Recommended Dilutions/Conditions: | Flow Cytometry (1:100)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~32kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation. |
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| Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis of frozen mouse spinal cord with HO-1, mAb (HO-1-2).
Immunohistochemistry analysis of frozen human prostate section (stressed) stained using HO-1 (Hsp32) mAb (HO-1-2).
Flow cytometry analysis of human lung cancer A2 cells stained using isotype control antibody (left) and HO-1 (Hsp32) mAb (HO -1-2) (right).
Western blot analysis: Lane 1: MW Marker, Lane 2: HO-1 Recombinant Rat Protein (Prod. No. ADI-SPP-730), Lane 3: Rat Liver Microsomes (Prod. No. ADI-LYT-RM100).
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Product Literature References
Mitochondrial Dysfunction Involved in the Cytotoxicity of Tramadol in Human Endometrial Carcinoma Cells: L.C. Liu, et al.; Int. J. Mol. Sci.
24, 99 (2023),
Abstract;
Physiological responses (Hsps 60 and 32, caspase 3, H2O2 scavenging, and photosynthetic activity) of the coral Pocillopora damicornis under thermal and high nitrate stresses: M. Thummasan, et al.; Mar. Pollut. Bull.
171, 112737 (2021),
Abstract;
Hormetic Effects of Binaphthyl Phosphonothioates as Pro-oxidants and Antioxidants: Y. Hirata, et al.; Chem. Res. Toxicol.
33, 2892 (2020),
Abstract;
Full Text
Haptoglobin and hemopexin inhibit vaso-occlusion and inflammation in murine sickle cell disease: Role of heme oxygenase-1 induction: J.D. Belcher, et al.; PLoS One
13, e0196455 (2018),
Abstract;
Full Text
Oral carbon monoxide therapy in murine sickle cell disease: Beneficial effects on vaso-occlusion, inflammation and anemia: J.D. Belcher, et al.; PLoS One
13, e0205194 (2018),
Abstract;
Full Text
Sexual dimorphism in the hepatic protein response to a moderate trans fat diet in senescence-accelerated mice: S.A. Bloomer, et al.; Lipids Health Dis.
16, 243 (2017),
Abstract;
Full Text
The cellular stress response of the scleractinian coral Goniopora columna during the progression of the black band disease: D. Seveso, et al.; Cell Stress Chaperones
22, 225 (2017),
Application(s): Western Blot,
Abstract;
Full Text
Hepatic Overexpression of Hemopexin Inhibits Inflammation and Vascular Stasis in Murine Models of Sickle Cell Disease: G.M. Vercellotti, et al.; Mol. Med.
22, 437 (2016),
Abstract;
Full Text
Anti-Apoptotic Effects of 3,3',5-Triiodo-L-Thyronine in the Liver of Brain-Dead Rats: R.A. Rebolledo, et al.; PLoS One
10, e0138749 (2015),
Application(s): Immunohistochemistry in rats,
Abstract;
Full Text
N,N-diethyldithiocarbamate promotes oxidative stress prior to myelin structural changes and increases myelin copper content: W. Valentine, et al. ; Toxicol. Appl. Pharmacol.
239, 71 (2009),
Application(s): WB using rat cell lysates,
Abstract;
Haem oxygenase-I gene transfer protects retinal ganglion cells from ischaemia/reperfusion injury: S. Juan, et al. ; Clin. Sci.
115, 335 (2008),
Application(s): WB using rat cell lysates,
Abstract;
Increased intestinal expression of heme oxygenase-1 and its localization in patients with ulcerative colitis: Y. Naito, et al. ; J. Gastroenterol. Hepatol.
23, S229 (2008),
Application(s): IHC using human tissue,
Abstract;
Statins attenuate ischemia-reperfusion injury by inducing hemeoxygenase-1 in infiltrating macrophages: F. Gueler, et al. ; Am. J. Pathol.
170, 1192 (2007),
Application(s): IHC using rat tissue,
Abstract;
Thiol antioxidants inhibit the adjuvant effects of aerosolized diesel exhaust particles in a murine model for ovalbumin sensitization: A.E. Nel, et al. ; J. Immunol.
168, 2560 (2002),
Application(s): WB using mouse samples,
Abstract;
Impact of hyperthermic preconditioning on postischemic hepatic microcirculatory disturbances in an isolated perfusion model of the rat liver: K. Messmer, et al. ; Hepatology
31, 407 (2000),
Application(s): WB using rat samples,
Abstract;
Overexpression of heme oxygenase-1 is neuroprotective in a model of permanent middle cerebral artery occlusion in transgenic mice: M.D. Maines, et al. ; J. Neurochem.
72, 1187 (1999),
Application(s): IHC using mouse samples,
Abstract;
Neuronal overexpression of heme oxygenase-1 correlates with an attenuated exploratory behavior and causes an increase in neuronal NADPH diaphorase staining: K.J. McCoubrey, et al. ; J. Neurochem.
70, 2057 (1998),
Application(s): WB using mouse samples,
Abstract;
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