Product Specification
| Alternative Name: | HMOX1, Hsp32, Heat shock protein 32, Heme oxygenase 1 |
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| Clone: | HO-1-1 |
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| Host: | Mouse |
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| Isotype: | IgG1κ |
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| Immunogen: | Synthetic peptide corresponding to the sequence near the N-terminus of human HO-1. |
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| UniProt ID: | P09601 |
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| GenBank ID: | X14782 |
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| Gene/Protein Identifier: | 3162 (Entrez GeneID), 141250 (OMIM) |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Dog
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| Applications: | Flow Cytometry, ICC, IHC (PS), WB
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| Recommended Dilutions/Conditions: | Flow Cytometry (10µg/ml)
Western Blot (1:1,000 ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~32kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation. |
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| Technical Info/Product Notes: | Recommended by the Human Protein Atlas Organization for IHC (Ensembl No. ENSG00000100292). |
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Western blot analysis of HO-1 (Hsp32), mAb (HO-1-1) (Prod. No. ADI-OSA-110): Lane 1: MW marker; Lane 2: HO-1 (rat), (recombinant) (Prod. No. ADI-SPP-730); Lane 3: HO-1 (human), (recombinant) (Prod. No. ADI-SPP-732); Lane 4: HO-2 (human), (recombinant) (Prod. No. ADI-SPP-550); Lane 5: MDBK cell lysate; Lane 6: Dog liver microsome; Lane 7: Mouse liver microsome.
Immunohistochemistry analysis of human spleen tissue stained with HO-1, mAb (HO-1-1) at 10µg/ml.
Flow cytometry analysis of 10^6 Jurkat cells stained using HO-1 monoclonal antibody (HO-1-1), (Prod. No. ADI-OSA-110), at a concentration of 50 µg/mL.
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Product Literature References
Acute glucose fluctuation impacts microglial activity, leading to inflammatory activation or self-degradation: C.F. Hsieh, et al.; Sci. Rep.
9, 840 (2019),
Application(s): WB - Human Brain Tissue,
Abstract;
Full Text
A preliminary attempt to establish multiple stress response profiles of human skin fibroblasts exposed to mild or severe stress during ageing in vitro: S.I.S. Ratten, et al.; Mech. Ageing Dev.
170, 92 (2018),
Abstract;
Cinnamic aldehyde inhibits vascular smooth muscle cell proliferation and neointimal hyperplasia in Zucker Diabetic Fatty rats: N.E. Buglak, et al.; Redox Biol.
19, 166 (2018),
Abstract;
Full Text
Dysregulated heme oxygenase-1low M2-like macrophages augment lupus nephritis via Bach1 induced by type I interferons: D. Kishimoto, et al.; Arthritis Res. Ther.
20, 64 (2018),
Abstract;
Full Text
Dysregulation of cardiac lipid parameters in high-fat high-cholesterol diet-induced rat model: Q. Han, et al.; Lipids Health Dis.
17, 255 (2018),
Abstract;
Full Text
Loss of heme oxygenase-1 accelerates mesodermal gene expressions during embryoid body development from mouse embryonic stem cells: Y.L. Lai, et al.; Redox Biol.
15, 51 (2018),
Abstract;
Full Text
Lutzomyia longipalpis Saliva Induces Heme Oxygenase-1 Expression at Bite Sites: N.F. Luz, et al.; Front. Immunol.
9, 2779 (2018),
Abstract;
Full Text
Neuroprotective effect of an Nrf2-ARE activator identified from a chemical library on dopaminergic neurons: Y. Izumi, et al.; Eur. J. Pharmacol.
818, 470 (2018),
Abstract;
Novel CIL-102 derivatives as potential therapeutic agents for docetaxel-resistant prostate cancer: D.R. Miller, et al.; Cancer Lett.
436, 96 (2018),
Abstract;
Propofol induces nuclear localization of Nrf2 under conditions of oxidative stress in cardiac H9c2 cells: T. Shinjo, et al.; PLoS One
13, e0196191 (2018),
Abstract;
Full Text
Inflammatory signature of cerebellar neurodegeneration during neonatal hyperbilirubinemia in Ugt1 -/- mouse model: S. Vodret, et al.; J. Neuroinflammation
14, 64 (2017),
Abstract;
Full Text
Molecular plasticity of vascular wall during N(G)-nitro-L-arginine methyl ester-induced hypertension: modulation of proinflammatory signals: W. Gonzalez, et al.; Hypertension
36, 103 (2017),
Application(s): IHC on Rat Aortic Tissue,
Abstract;
Full Text
N-3 PUFAs induce inflammatory tolerance by formation of KEAP1-containing SQSTM1/p62-bodies and activation of NFE2L2: J. Mildenberger, et al.; Autophagy
13, 1664 (2017),
Abstract;
Full Text
Nrf2- and Bach1 May Play a Role in the Modulation of Ultraviolet A-Induced Oxidative Stress by Acetyl-11-Keto-β-Boswellic Acid in Skin Keratinocytes: S. Yang, et al.; Skin Pharmacol. Physiol.
30, 13 (2017),
Abstract;
Phosphorylation of eIF2α suppresses cisplatin-induced p53 activation and apoptosis by attenuating oxidative stress via ATF4-mediated HO-1 expression in human renal proximal tubular cells: S.M. Ju, et al.; Int. J. Mol. Med.
40, 1957 (2017),
Abstract;
Protective effect of Nrf2-ARE activator isolated from green perilla leaves on dopaminergic neuronal loss in a Parkinson's disease model: Y. Masaki, et al.; Eur. J. Pharmacol.
798, 26 (2017),
Abstract;
Treatment of acute fibrinous organizing pneumonia following hematopoietic cell transplantation with etanercept: G.L. Simmons, et al.; Bone Marrow Transplant.
52, 141 (2017),
Application(s): Lung Biopsy with pathology of AFOP,
Abstract;
Full Text
A small-molecule Nrf1 and Nrf2 activator mitigates polyglutamine toxicity in spinal and bulbar muscular atrophy: L.C. Bott, et al.; Hum. Mol. Genet.
25, 1979 (2016),
Abstract;
Full Text
Delivery of Soluble Heme Oxygenase 1 Cell-Penetrating Peptide into Liver Cells in in vitro and ex vivo Models of Cold Ischemia: A.B. Venkatachalam, et al.; Eur. Surg. Res.
58, 51 (2016),
Abstract;
DHA-induced stress response in human colon cancer cells-focus on oxidative stress and autophagy: K. Pettersen, et al.; Free Radic. Biol. Med.
90, 158 (2016),
Application(s): Cell culture,
Abstract;
Heme Oxygenases in Cardiovascular Health and Disease: A. Ayer, et al.; Physiol. Rev.
96, 1449 (2016),
Abstract;
Full Text
Increased iron export by ferroportin induces restriction of HIV-1 infection in sickle cell disease: N. Kumari, et al.; Blood Adv.
1, 170 (2016),
Abstract;
Full Text
Intracellular glutathione determines bortezomib cytotoxicity in multiple myeloma cells: K.K. Starheim, et al.; Blood Cancer J.
6, e446 (2016),
Application(s): Reagent, gene expression analysis,
Abstract;
Full Text
Knockout of ho-1 protects the striatum from ferrous iron-induced injury in a male-specific manner in mice: L.F. Wang, et al.; Sci. Rep.
6, 26358 (2016),
Abstract;
Full Text
The protective mechanism of docosahexaenoic acid in mouse model of Parkinson: The role of heme oxygenase: A. Ozkan, et al.; Neurochem. Int.
101, 110 (2016),
Application(s): Western blot analysis, mouse SN tissues,
Abstract;
Autophagy Deficiency Diminishes Indomethacin-Induced Intestinal Epithelial Cell Damage through the Activation of ERK/Nrf2/HO-1 Pathway: S. Harada, et al.; J. Pharmacol. Exp. Ther.
355, 353 (2015),
Application(s): Cell Culture,
Abstract;
Full Text
Cytotoxic Effects of Tropodithietic Acid on Mammalian Clonal Cell Lines of Neuronal and Glial Origin: H. Wichmann, et al.; Mar. Drugs.
13, 7113 (2015),
Application(s): Immunoblot analysis,
Abstract;
Full Text
Effect of soluble guanylyl cyclase activator and stimulator therapy on nitroglycerin-induced nitrate tolerance in rats: A. Jabs, et al.; Vascul. Pharmacol.
71, 181 (2015),
Application(s): Western blot,
Abstract;
Gastroprotective effect of orexin-A and heme oxygenase system: B. Gemici, et al.; J. Surg. Res.
193, 626 (2015),
Application(s): Western blot using rat tissue samples,
Abstract;
Heme oxygenase-1 ameliorates kidney ischemia-reperfusion injury in mice through extracellular signal-regulated kinase 1/2-enhanced tubular epithelium proliferation: H. Chen, et al. ; Biochim. Biophys. Acta
1852, 2195 (2015),
Application(s): Western blot ,
Abstract;
Heme Oxygenase-1 Protects Endothelial Cells from the Toxicity of Air Pollutant Chemicals: A. Lawal, et al.; Toxicol. Appl. Pharmacol.
284, 281 (2015),
Application(s): Western Blotting,
Abstract;
Nitration of indoxyl sulfate facilitates its cytotoxicity in human renal proximal tubular cells via expression of heme oxygenase-1: Y. Ishima, et al.; Biochem. Biophys. Res. Commun.
465, 481 (2015),
Application(s): Western blot,
Abstract;
The marine n-3 PUFA DHA evokes cytoprotection against oxidative stress and protein misfolding by inducing autophagy and NFE2L2 in human retinal pigment epithelial cells: I. Johansson, et al.; Autophagy
11, 1636 (2015),
Abstract;
Full Text
HER2 confers drug resistance of human breast cancer cells through activation of NRF2 by direct interaction: H.J. Kang, et al.; Sci. Rep.
4, 7201 (2014),
Application(s): Western blot using human breast cancer cells MCF7,
Abstract;
Full Text
Impact of aging on heat shock protein expression in the substantia nigra and striatum of the female rat: A.M. Gleixner, et al.; Cell Tissue Res.
357, 43 (2014),
Application(s): Western blot using rat substantia nigra and striatium tissue samples,
Abstract;
Red blood cells upregulate cytoprotective proteins and the labile iron pool in dividing human T cells despite a reduction in oxidative stress: F.A. Arosa, et al. ; Free Radic. Biol. Med.
35, 1404 (2003),
Application(s): WB using human samples,
Abstract;
Defective hematopoiesis and hepatic steatosis in mice with combined deficiencies of the genes encoding Fancc and Cu/Zn superoxide dismutase: S. Hadjur, et al.; Blood
98, 1003 (2001),
Application(s): WB using mouse samples,
Abstract;
Hmox-1 constitutes an adaptive response to effect antioxidant cardioprotection: a study with transgenic mice heterozygous for targeted disruption of the Heme oxygenase-1 gene: D.K. Das, et al. ; Circulation
103, 1695 (2001),
Application(s): WB using mouse samples,
Abstract;
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