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Neurofilament M monoclonal antibody (3H11)

 
ADI-NBA-140-E 100 µl 273.00 USD
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Product Specification

Alternative Name:NEFM, NF-M
 
Clone:3H11
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant rat neurofilament M (NF-M) (C-terminal fragment).
 
UniProt ID:P12839
 
Species reactivity:Human, Mouse, Rat
Chicken
 
Applications:ICC, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:25-1:100)
Western Blot (1:200-1:1,000 ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol, 0.01% thimerosal, and 1.0mg/ml BSA.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Neuron-specific neurofilaments belong to the intermediate filament protein family, also known as 10nm filaments. As major constituents of the axonal cytoskeleton, neurofilaments contain heteropolymers of three major proteins: 200 kDa heavy molecular weight protein NF-H, 160 kDa medium molecular weight protein NF-M, and 68 kDa light molecular weight protein NF-L, with the ratio of each changing during neuronal development. All three proteins contain globular head and tail domains flanking a central alpha-helical domain. An accumulation of neurofilament proteins and/or mutations in one of the three major proteins occur during the progression of numerous neurological diseases, including ALS.
 
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