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[pSer8]GFAP monoclonal antibody (YC10)

 
ADI-NBA-115-D 50 µg 281.00 USD
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Product Specification

Alternative Name:Glial fibrillary acidic protein
 
Clone:YC10
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to the N-terminus of pig GFAP phosphorylated at Ser8.
 
UniProt ID:P14136 (human)
 
Species reactivity:Human
Bovine, Porcine
 
Applications:ELISA, ICC, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (10µg/ml)
Kinase Activity Assay (1µg/ml)
Western Blot (1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~50kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Glial Fibrillary Acidic Protein (GFAP) is an intermediate-filament (IF) protein that is specific for cells of astroglial lineage. The major IF protein expressed in precurosor astrocytes in the CNS is usually vimentin. A switch between vimentin and GFAP expression occurs following astrocyte maturation, making GFAP a widely used marker for astrocyte maturation. GFAP expression is modulated by several hormones including thyroid hormone, glucocorticoids and several growth factors such as FGF, CNTF, and TGF-beta.
 

Product Literature References

Two different protein kinases act on a different time schedule as glial filament kinases during mitosis: M. Inagaki, et al. ; EMBO J. 11, 2895 (1992), Application(s): WB, ICC using human samples, Abstract;
A monoclonal antibody to the phosphorylated form of glial fibrillary acidic protein: application to a non-radioactive method for measuring protein kinase activities: M. Inagaki, et al. ; Biochem. Biophys. Res. Commun. 175, 1144 (1991), Application(s): WB, EIA using bovine, porcine & human samples, Abstract;

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