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RecA (E. coli) monoclonal antibody (ARM193)

 
ADI-MSA-205-E 100 µg 313.00 USD
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Product Specification

Clone:ARM193
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:E. coli RecA.
 
UniProt ID:P0A7G6
 
Species reactivity:E. coli
 
Applications:WB
Inhibition assay
 
Recommended Dilutions/Conditions:Western Blot (1µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Inhibition Assay (Equal to RecA protein). Detects a band of ~40kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Lyophilized. In PBS, pH 7.2, containing 1% sucrose and 0.09% sodium azide. For a stock solution of 1.0mg/ml reconstitute with 100μl of sterile distilled water.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The RecA protein is a member of the RecA/Rad51 family of proteins which are ubiquitous among organisms from bacteria to humans. RecA protein of E. coli is a catalyst of DNA strand exchange in the early phase of homologous recombination involving the exchange of two aligned, nearly identical strands of DNA. RecA is also a key enzyme in recombinational repair of damaged DNA.
 
MSA-205 WB
Western blot analysis: RecA expression in BB4 E. coli lysate using RecA, mAb (ARM193).
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MSA-205 WB

Product Literature References

Type III Secretion-Dependent Sensitivity of Escherichia coli O157 to Specific Ketolides: R.J. Fernandez-Brando, et al.; Antimicrob Agents Chemother. 60, 459 (2015), Application(s): Western blot, Abstract; Full Text

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