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BrdU monoclonal antibody (2B1)

 
ADI-MSA-200-E 100 µg 301.00 USD
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Product Specification

Alternative Name:5-Bromodeoxyuridine
 
Clone:2B1
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Iododeoxyuridine-Ovalbumin.
 
Species reactivity:Species independent
 
Applications:Flow Cytometry, IHC
 
Recommended Dilutions/Conditions:Flow Cytometry (10µg/ml)
Immunohistochemistry (10µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The thymidine analogue BrdU (5-Bromo-2-Deoxyuridine), a derivative of uridine, can substitute for thymidine during DNA synthesis. The detection of BrdU incorporation into DNA provides a common method to quantify newly synthesized DNA and identify cells in the S-phase of the cell cycle. The use of BrdU incorporation in proliferation assays facilitates the study of DNA repair, sister chromatid exchange, and the cytokinetics of normal and neoplastic cells.
 
MSA-200 IHC
Immunohistochemistry analysis of paraffin-embedded section of mouse testis stained with BrdU, mAb (2B1) to detect cells which have incorporated BrdU.
MSA-200 FC
Flow cytometric analysis indicates the reaction of isotypic control (open histogram) and BrdU, mAb (2B1) (shaded histogram) to BrdU treated Raji cells.
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MSA-200 IHC MSA-200 FC

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