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Myc-tag monoclonal antibody (PL14)

 
ADI-MSA-110-F 200 µg 345.00 USD
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Product Specification

Clone:PL14
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:6myc-tag GST fusion protein.
 
UniProt ID:P01106 (human)
 
Specificity:Recognizes the Myc-tag of Myc-tagged recombinant proteins.
 
Applications:ICC, IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (2µg/ml)
Immunoprecipitation (5µg/ml)
Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol. No preservative has been added.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Epitope tagging is a widely accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, gene expression can be easily monitored on Western blotting, immunoprecipitation and immunofluorescence utilizing an antibody that recognizes such an epitope. The Myc-Tag (EQKLISEEDL) corresponds to the partial peptide sequence of the human c-myc gene product.
 
MSA-110 WB
Western blot analysis of 293T cells transfected with 6Myc/Heparanase (lane 1) and 6Myc/cdc25 (lane 2), probed with Myc-tag mAb (PL14).
MSA-110 ICC
Immunocytochemistry analysis of monkey Cos-7 cells transfected with 6myc-hCAP1, stained with Myc-tag mAb (PL14).
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MSA-110 WB MSA-110 ICC

Product Literature References

ZnT7, a novel mammalian zinc transporter, accumulates zinc in the Golgi apparatus: L. Huang, et al. ; J. Biol. Chem. 278, 4096 (2003), Application(s): ICC using human samples, Abstract;
Functional characterization of a novel mammalian zinc transporter, ZnT6: J. Gitschier, et al. ; J. Biol. Chem. 277, 26389 (2002), Application(s): WB using human samples, Abstract;

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