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Heart (mouse), (tissue extract)

 
ADI-LYT-MH100-0200 200 µg 103.00 USD
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Product Specification

Recommended Dilutions/Conditions:Western Blot (20µg/lane)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Source:Mouse heart tissue extract.
 
Formulation:Liquid. In SDS-PAGE sample buffer, pH 6.8, containing 40mM TRIS-HCl, 1% SDS, 50mM DTT, 7.5% glycerol, and 0.003% bromophenol blue.
 
Applications:WB
 
Application Notes:Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:Hearts from Swiss Webster mice, mixed gender, were suspended in M-PER, Mammalian Protein Extraction Reagent, with protease inhibitor cocktail and homogenized using a Dounce tissue grinder. The tissue lysate was adjusted to 2mg/mL in SDS PAGE sample buffer (40 mM Tris-HCl pH 6.8, 1% SDS, 50 mM DTT, 7.5% glycerol, 0.003% bromophenol blue) and heated for 10 min at 70°C.
 

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