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PC-12, (cell lysate)

 
ADI-LYC-PC100-F 200 µg 109.00 USD
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Product Specification

Source:Rat
 
Concentration:2mg/ml
 
Formulation:Liquid. In SDS-PAGE sample buffer, pH 6.8, heated for 10 min. at 70°C and containing 40mM TRIS-HCl, 1% SDS, 50mM DTT, 7.5% glycerol, and 0.003% bromophenol blue.
 
Applications:WB
 
Application Notes:Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:PC-12 cells were maintained in Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 82.5%; horse serum, 15%; fetal bovine serum, 2.5%. Cells were collected from media by centrifuging at 1200 rpm at 4°C in a Beckman GS-6R centrifuge for 7 minutes. Cells were washed twice in PBS and were lysed in M-PER Mammalian Protein Extraction Reagent (Pierce catalog #78501) with a protease inhibitor cocktail. Cell lysate was adjusted to 2mg/mL in SDS PAGE sample buffer (40 mM Tris-HCl, pH 6.8, 1% SDS, 50 mM DTT, 7.5% glycerol, 0.003% Bromophenol blue), and heated for 10 min at 70°C.
 

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