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HeLa (heat shocked) , (cell lysate)

ADI-LYC-HL101-F 200 µg 111.00 USD
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Product Specification

Recommended Dilutions/Conditions:Western Blot (20µg/lane)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Formulation:Liquid. In SDS-PAGE sample buffer.
Application Notes:Western blot control.
Shipping:Shipped on Dry Ice
Long Term Storage:-80°C
Handling:Avoid freeze/thaw cycles.
Scientific Background:The HeLa cell line was originally derived from a human donor's epithelioid carcinoma tissue (cervix). Research into the heat shock response provides a model for studying the effects of environmental and homeostatic stressors upon cells. Heat shocking reproducibly alters gene expression in cells, resulting in various morphological changes and the expression of heat shock proteins crucial for both maintaining cellular homeostasis during normal cell growth and for survival during and after various cellular stresses. Heat shocking increases morphological changes compatible with apoptosis in many cell types. In mammalian cells, the induction of the heat shock response requires the activation and nuclear translocation of transcription factors which control the expression of cytoprotective heat shock proteins, and play pivotal roles in the development of inflammation and immunity.
SDS-Page Analysis: Lane 1: MW marker; Lane 2: HeLa cell lysate heat shocked (10µg); and Lane 3: HeLa cell lysate heat shocked (20µg).
Western blot analysis: Lane 1: MW marker; Lane 2: HeLa cell lysate control (10µg); Lane 3: HeLa cell lysate control (20µg); Lane 4: HeLa cell lysate heat shocked (10µg); and Lane 5: HeLa cell lysate heat shocked (20µg).
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Product Literature References

Effects of feed restriction on the upper temperature tolerance and heat shock response in juvenile green and white sturgeons: S. Lee, et al.; Comp. Biochem. Physiol. A Mol. Integr. Physiol. 198, 87 (2016), Application(s): Internal standard, Abstract;
Probing the phenomenon of trained immunity in invertebrates during a transgenerational study, using brine shrimp Artemia as a model system: P. Norouzitallab, et al.; Sci. Rep. 6, 21166 (2016), Application(s): Western blot, Abstract; Full Text
The gnotobiotic brine shrimp (Artemia franciscana) model system reveals that the phenolic compound pyrogallol protects against infection through its prooxidant activity: K. Baruah, et al.; Free Radic. Biol. Med. 89, 593 (2015), Application(s): Positive control in Protein extraction and Hsp70 detection, Abstract;
Superoxide during reperfusion contributes to caspase-8 expression and apoptosis after transient focal stroke: P.H. Chan, et al. ; Stroke 32, 2356 (2001), Application(s): WB , Abstract;

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