HeLa (heat shocked) , (cell lysate)



ADI-LYC-HL101-F	200 µg	95.00 USD

Product Specification

Formulation:	Liquid. In SDS-PAGE sample buffer (40mM TRIS-HCl, pH 8.8, containing 1% SDS, 50mM DTT, 7.5% glycerol, 0.003% Bromophenol Blue).

Source/Host:	Human (cervical adenocarcinoma cells (He La), heat shocked

Application:	Western Blot (control, 20µg/lane) Optimal conditions must be determined individually for each application.

Long Term Storage:	-80°C

Miscellaneous/General:	The HeLa cell line was originally derived from a human donor's epithelioid carcinoma tissue (cervix). Research into the heat shock response provides a model for studying the effects of environmental and homeostatic stressors upon cells. Heat shocking reproducibly alters gene expression in cells, resulting in various morphological changes and the expression of heat shock proteins crucial for both maintaining cellular homeostasis during normal cell growth and for survival during and after various cellular stresses. Heat shocking increases morphological changes compatible with apoptosis in many cell types. In mammalian cells, the induction of the heat shock response requires the activation and nuclear translocation of transcription factors which control the expression of cytoprotective heat shock proteins, and play pivotal roles in the development of inflammation and immunity.

SDS-Page Analysis: Lane 1: MW marker; Lane 2: HeLa cell lysate heat shocked (10µg); and Lane 3: HeLa cell lysate heat shocked (20µg).

Western blot analysis: Lane 1: MW marker; Lane 2: HeLa cell lysate control (10µg); Lane 3: HeLa cell lysate control (20µg); Lane 4: HeLa cell lysate heat shocked (10µg); and Lane 5: HeLa cell lysate heat shocked (20µg).

Please mouse over

Product Literature References

Superoxide during reperfusion contributes to caspase-8 expression and apoptosis after transient focal stroke: P.H. Chan, et al. ; Stroke 32, 2356 (2001), Application(s): WB , Abstract;