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A431 (TSA treated), (cell lysate)

 
ADI-LYC-A4102-F 200 µg 94.00 USD
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Product Specification

Recommended Dilutions/Conditions:Western Blot (20µg/lane)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Source:Human
 
Formulation:Liquid. In SDS-PAGE sample buffer, pH 6.8, heated for 10 min. at 70°C and containing 40mM TRIS-HCl, 1% SDS, 50mM DTT, 7.5% glycerol, and 0.003% bromophenol blue.
 
Applications:WB
 
Application Notes:Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:The A431 cell line is derived from a human epithelial carcinoma, and is commonly used as a positive control for the study of growth factor action and binding due to its high expression of EGFR. Trichostatin A (TSA) is an antifungal antibiotic isolated from Streptomyces that functions as a potent and specific inhibitor of mammalian histone deacetylase (HDAC). Treatment of A431 cells with TSA leads to the accumulation of acetylated proteins including histones and heat shock protein 90 (Hsp90).
 

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