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3T3 (heat shocked) , (cell lysate)

 
ADI-LYC-3T101-F 200 µg 109.00 USD
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Product Specification

Recommended Dilutions/Conditions:Western Blot (20µg/lane)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Source:Mouse
 
Concentration:2mg/ml
 
Formulation:Liquid. In SDS-PAGE sample buffer, pH 8.8, heated for 10 min. at 70°C and containing 40mM TRIS-HCl, 1% SDS, 50mM DTT, 7.5% glycerol, and 0.003% bromophenol blue.
 
Applications:WB
 
Application Notes:Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:3T3 cells were maintained in Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; bovine calf serum, 10%. 3T3 cells were heat shocked at 44°C for 2 hours and allowed to recover for five to eighteen hours at 37°C before harvesting. Cells were collected from media by centrifuging at 1200 rpm at 4°C in a Beckman GS-6R centrifuge for 7 minutes. Cells were washed twice in PBS and were lysed in M-PER Mammalian Protein Extraction Reagent (Pierce catalog# 78501) with a protease inhibitor cocktail. The cell lysate was adjusted to 2mg/mL in SDS PAGE sample buffer (40 mM Tris-HCl pH 6.8, 1% SDS, 50 mM DTT, 7.5% glycerol, 0.003% bromophenol blue) and heated for 10 min at 70°C.
 

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