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eIF2α (human), (recombinant) (His-tag)

 
ADI-KPR-CP132-0050 50 µg 194.00 USD
 
ADI-KPR-CP132-0200 200 µg 465.00 USD
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Product Specification

Alternative Name:Eukaryotic translation initiation factor 2 subunit α
 
MW:~36kDa
 
Source:Produced in E. coli. Human eIF2α is fused at the N-terminus to a His-tag.
 
UniProt ID:P05198
 
Formulation:Liquid. In 50mM TRIS, pH 7.5, containing 10% glycerol, 0.5% Triton X-100, 300mM potassium chloride, and 4mM DTT.
 
Purity:≥85% (SDS-PAGE; Western blot)
 
Purity Detail:Purified by multi-step chromatography.
 
Applications:WB
 
Application Notes:Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:Eukaryotic translation initiation factor 2α (eIF2α) promotes the binding of initiator tRNA to the 40S ribosomal subunits, and controls translational rates via phosphorylation/dephosphorylation mechanisms. The double-stranded RNA (dsRNA)-activated protein kinase (PKR) provides an important control in the regulation of protein synthesis initiation through the phosphorylation of eIF2α. Phosphorylated eIF2α inhibits cell growth and induces apoptosis. Phosphorylation of eIF2α occurs under a variety of conditions, including viral infection, apoptosis, nutrient deprivation, heme-deprivation, and certain stresses. eIF2 exists as a heterotrimeric protein composed of 3 nonidentical subunits: α (36kDa), β (38kDa), and γ (52kDa).
 
KPR-CP132 SDS-PAGE
SDS-PAGE Analysis of eIF2α (human), (recombinant) (His-tag): Lane 1: MW Marker, Lane 2: 0.5μg, Lane 3: 1.0μg, Lane 4: 2.0μg, Lane 5: 3.0μg.
KPR-CP132 WB
Western Blot Analysis of eIF2α (human), (recombinant) (His-tag) probed with eIF2α polyclonal antibody: Lane 1: MW Marker, Lane 2: 200ng, Lane 3: 400ng.
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KPR-CP132 SDS-PAGE KPR-CP132 WB

Product Literature References

Chronic Hyperinsulinemia increases myoblast proliferation in fetal sheep skeletal muscle: L.D. Brown, et al.; Endocrinology 157, 2447 (2016), Application(s): Western blot, Abstract; Full Text

General Literature References

Double-stranded RNA-activated protein kinase (PKR) is negatively regulated by 60S ribosomal subunit protein L18: K.U. Kumar, et al.; Mol. Cell. Biol. 19, 1116 (1999), Abstract;
Effect of brain ischemia and reperfusion on the localization of phosphorylated eukaryotic initiation factor 2 alpha: D.J. DeGracia, et al.; J. Cereb. Blood Flow Metab. 17, 1291 (1997), Abstract;
Localization of eukaryotic initiation factor 2 in neuron primary cultures and established cell lines: M.V. Lobo, et al.; Histochem. J. 29, 453 (1997), Abstract;

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