Product Details
Alternative Name: | NOS2, NOS II, Nitric oxide synthase (inducible), Hepatocyte NOS |
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Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to aa 1131-1144 of mouse iNOS. |
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UniProt ID: | P29477 |
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Gene/Protein Identifier: | NM_010927 (RefSeq) |
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Source: | Purified from rabbit serum. |
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Species reactivity: | Mouse, Rat Bovine
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Applications: | IP, WB
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Recommended Dilutions/Conditions: | Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~130kDa by Western blot. |
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Purity Detail: | Protein A affinity purified. |
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Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | The diffusible free radical gas nitric oxide (NO) affects a variety of physiological functions, and is a key regulator of the cardiovascular, nervous, and immune systems. NO is synthesized in many tissues from L-arginine, oxygen, and NADPH by three known isoforms of a heme-containing flavoprotein termed NO synthase (nNOS/NOS-I, iNOS/NOS-II, and eNOS/NOS-III). nNOS is a constitutively expressed neuronal NOS isoform that exits in its latent form until it is activated by the binding of calmodulin follo wing elevation of intracellular calcium levels. The C-terminus of nNOS contains a conserved serine residue, Ser1417, analogous to Ser1177 of the constitutively expressed endothelial NOS isoform (eNOS). Phosphorylation of Ser1417 is believed to regulate nNOS activation, particularly in glucose-sensing neurons, where inhibition of AMPK pathways by glucose and leptin serve to suppress nNOS activity, whereas activation of AMPK by insulin leads to nNOS activation. |
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Regulatory Status: | RUO - Research Use Only |
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Western blot analysis: rat brain extract, probed with iNOS, pAb.
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Product Literature References
Enteric neuroanatomy and smooth muscle activity in the western diamondback rattlesnake (Crotalus atrox): T. Kohl, et al.; Front. Zool.
20, s12983 (2023),
Abstract;
C57BL/6N Mice Are More Resistant to Ehrlich Ascites Tumors Than C57BL/6J Mice: The Role of Macrophage Nitric Oxide: S. Kalish, et al.; Med. Sci. Monit. Basic Res.
21, 235 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Dietary Supplementation of Genistein Attenuates Inflammatory Responses and Oxidative Stress during Cutaneous Wound Healing in Diabetic Mice: H. Eo, et al.; J. Agr. Sci.
7, 80 (2015),
Application(s): Western Blotting,
Full Text
Phenolic content of extra virgin olive oil is essential to restore endothelial dysfunction but not to prevent vascular inflammation in atherosclerotic lesions of Apo E deficient mice: C. Claro, et al.; J. Funct. Foods 15, 126 (2015), Application(s): Western Blot,
Protective effect of a novel Rho kinase inhibitor WAR-5 in experimental autoimmune encephalomyelitis by modulating inflammatory response and neurotrophic factors: Y.H. Li, et al.; Exp. Mol. Pathol.
99, 220 (2015),
Application(s): Injection into mouse abdominal cavity,
Abstract;
Proapoptotic nitric oxide production in amyloid β protein-treated cerebral microvascular endothelial cells: M. Oike, et al. ; Microcirculation
14, 89 (2007),
Application(s): WB using bovine cell lysates,
Abstract;
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