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Methylated Lysine polyclonal antibody

 
ADI-KAP-TF121-E 100 µg 319.00 USD
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Product Specification

Host:Rabbit
 
Immunogen:Methylated KLH.
 
Species reactivity:Species independent
 
Applications:ELISA, IHC, WB
 
Recommended Dilutions/Conditions:Western Blot (0.25-1.0µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Immunoaffinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Acetylation and methylation of lysine are important post-translational modifications that regulate numerous protein-protein and protein-DNA interactions. Lysine acetylation and methylation involves the transfer of acetylCoA, or one or more methyl groups, to the e-amino group of lysine by modifying enzymes and cofactors. Histones and transcription factors are the primary targets of lysine acetylation and methylation, with either modification capable of inducing gene silencing or expression due to differential regulation of cofactors. For example, varying degrees of mono-, di-, and tri-methylation or acetylation of histone H3 at lysine residue 9 are known to demark distinct chromatin regions during various states of gene activation (methylation) or repression (acetylation).
 
KAP-TF121 WB
Western blot analysis of melanoma cells with Methylated Lysine pAb. Histones were isolated with cold 0.1N HCl extraction, then probed with 0.5µg/ml of Methylated Lysine pAb in TBSt and blocked with synthetic methylated BSA 100µg/ml (left). And 0.5µg/ml of Methylated Lysine pAb in TBSt (right).
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KAP-TF121 WB

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Lysine
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Lysine Polyclonal antibody
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