Acetylated Lysine, pAb



ADI-KAP-TF120-E	100 µg	240.00 USD

Product Specification

Purity Detail:	Protein A affinity purified.

Formulation:	Liquid. In PBS, pH 7.0, containing 0.09% sodium azide.

Immunogen:	Acetylated KLH.

Source/Host:	From rabbit.

Specificity:	Species independent.

Application:	ELISA (1:1000) Immunofluorescence (1:50) Immunoprecipitation (1:25) Western Blot (1:250) Optimal conditions must be determined individually for each application.

Long Term Storage:	-20°C

Miscellaneous/General:	Acetylation and methylation of lysine are important post-translational modifications that regulate numerous protein-protein and protein-DNA interactions. Lysine acetylation and methylation involves the transfer of acetylCoA, or one or more methyl groups, to the e-amino group of lysine by modifying enzymes and cofactors. Histones and transcription factors are the primary targets of lysine acetylation and methylation, with either modification capable of inducing gene silencing or expression due to differential regulation of cofactors. For example, varying degrees of mono-, di-, and tri-methylation or acetylation of histone H3 at lysine residue 9 are known to demark distinct chromatin regions during various states of gene activation (methylation) or repression (acetylation).

Immunofluorescent analysis of TSA treated (right - 100ng/ml, 24 hours) and non-treated (left) human MMRU melanoma cells using Acetylated Lysine (RS2) pAb.

Immunoprecipitation analysis of acetylated BSA with Acetylated Lysine (RS1) pAb in ng.

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Product Literature References

Histone deacetylase inhibitors synergize p300 autoacetylation that regulates its transactivation activity and complex formation: N. Sang, et al. ; Cancer Res. 67, 2256 (2007), Application(s): NA using human cell lysates, Abstract;