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Casein Kinase 2 α polyclonal antibody

ADI-KAP-ST010-E 100 µg 296.00 USD
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Product Specification

Alternative Name:CSNK, CK
Immunogen:Synthetic peptide corresponding to a portion of human CK2α (casein kinase 2α). The sequence is completely conserved in mouse, rat, rabbit, chicken, Xenopus, zebra fish, Drosophila and C. elegans.
UniProt ID:P68400 (CK2 alpha), P19784 (CK2 alpha')
GenBank ID:X70251 (CK2 alpha), M55268 (CK2 alpha')
Species reactivity:Human, Mouse, Rat
Bovine, Chinook salmon, Drosophila, Guinea pig, Monkey, Rabbit, Sheep
Applications:IF, IHC, WB
Recommended Dilutions/Conditions:Immunohistochemistry (1.7-8.5µg/ml)
Western Blot (4µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects band of ~41kDa and ~45kDa by Western blot. An additional band with an apparent molecular mass of ~38 kDa is detected in brain and heart tissue extracts.
Purity Detail:Immunoaffinity purified.
Formulation:Liquid. In borate buffered saline, pH 8.2, containing 25mM sodium borate, 100mM boric acid, 75mM sodium chloride, and 5mM EDTA (disodium salt).
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:The casein kinases are serine/threonine protein kinases involved in the regulation of multiple cell signaling pathways. There are at least seven identified mammalian isoforms in the casein kinase I (CKI) family, which are monomeric and function in cell growth and the replication and repair of DNA. Casein kinase II (CK2) is a ubiquitous tetrameric holoenzyme comprised of two catalytic alpha subunits and two regulatory beta subunits. CK2 enzymes are known to regulate metabolic signal transduction, transcription, and translation.
Proliferating human Caco-2 cells stained with Casein Kinase 2 alpha polyclonal antibody (Prod. No. ADI-KAP-ST010).
Western blot analysis of mouse spleen tissue extract, probed with Casein Kinase 2 alpha polyclonal antibody (Prod. No. ADI-KAP-ST010).
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Product Literature References

Dysregulation of integrin-linked kinase (ILK) signaling in colonic polyposis: B. Salh, et al. ; Oncogene 20, 6250 (2003), Application(s): WB using human samples, Abstract;
A role for casein kinase 2alpha in the Drosophila circadian clock: R. Allada, et al. ; Nature 420, 816 (2002), Application(s): IHC using drosophila samples, Abstract;
Functional interaction of protein kinase CK2 and c-Myc in lymphomagenesis: D.C. Seldin, et al. ; Oncogene 21, 5280 (2002), Application(s): WB using mouse samples, Abstract;
Heat-shock protein90 complexes in resting and thrombin-activated platelets: R.L. Gear, et al. ; Biochem. Biophys. Res. Commun. 297, 129 (2002), Application(s): WB using human samples, Abstract;

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