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PKG polyclonal antibody

ADI-KAP-PK005-D 50 µg 159.00 USD
ADI-KAP-PK005-F 200 µg 363.00 USD
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Product Specification

Alternative Name:Protein kinase G
Immunogen:Synthetic peptide corresponding to a portion of human PKG.
UniProt ID:Q13976
GenBank ID:D45864
Species reactivity:Human, Mouse, Rat
Applications:IHC (PS), WB
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~75kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Cyclic GMP-dependent protein kinases (PKGs or cGKs) are classified into two types, PKGI and PKGII. Studies have shown that PKGs are highly homologous to PKAs; phosphorylation of cellular proteins by both families of kinases leads to alterations in calcium mobilization, protein phosphatase activity, ion channel function, gene transcription, smooth muscle contractility, and platelet aggregation.
Western blot analysis: Lane 1: MW marker, Lane 2: Mouse Brain Tissue Extract, Lane 3: Rat Brain Tissue Extract, Lane 4: EKS4 Cell Lysate; Lane 5: HS67 Cell Lysate probed with PKG pAb.
Immunohistochemistry analysis of human prostate tissue stained with PKG, pAb at 10µg/ml.
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Product Literature References

Age related differences in responsiveness to sildenafil and tamsulosin are due to myogenic smooth muscle tone in the human prostate: S.N. Lee, et al.; Sci. Rep. 7, 10150 (2017), Application(s): Immunohistochemistry, Abstract; Full Text
Examining a role for PKG Iα oxidation in the pathogenesis of cardiovascular dysfunction during diet-induced obesity: O. Rudyk, et al.; Free Radic. Biol. Med. 110, 390 (2017), Application(s): Western blot, Abstract; Full Text
Oxidant sensor in the cGMP-binding pocket of PKGIα regulates nitroxyl-mediated kinase activity: S. Donzelli, et al.; Sci. Rep. 7, 9938 (2017), Application(s): Western blot, Abstract; Full Text
Type 5 phosphodiesterase regulates glioblastoma multiforme aggressiveness and clinical outcome: V. Cesarini, et al.; Oncotarget 8, 13223 (2017), Application(s): Immunohistochemistry, Abstract; Full Text
Disulfide-activated protein kinase G Iα regulates cardiac diastolic relaxation and fine-tunes the Frank-Starling response: J. Scotcher, et al.; Nat. Commun. 7, 13187 (2016), Application(s): Western blot, Abstract; Full Text
Phosphodiesterase 5 inhibition limits doxorubicin-induced heart failure by attenuating protein kinase G Iα oxidation: O. Prysyazhna, et al.; J. Biol. Chem. 291, 17427 (2016), Application(s): Western blot, Abstract; Full Text
cCMP causes caspase-dependent apoptosis in mouse lymphoma cell lines: S. Wolter, et al.; Biochem. Pharmacol. 98, 119 (2015), Application(s): Western blot, Abstract;
Crystal Structures of the Carboxyl cGMP Binding Domain of the Plasmodium falciparum cGMP-dependent Protein Kinase Reveal a Novel Capping Triad Crucial for Merozoite Egress: J.J. Kim, et al.; PLoS Pathog. 11, e1004639 (2015), Application(s): Western Blotting, Abstract; Full Text
The soluble guanylate cyclase activator cinaciguat prevents cardiac dysfunction in a rat model of type-1 diabetes mellitus: C. Mátyás, et al.; Cardiovasc. Diabetol. 14, 145 (2015), Application(s): Immunoblot analysis, Abstract; Full Text
Proprotein convertases play an important role in regulating PKGI endoproteolytic cleavage and nuclear transport: S. Kato, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 305, L130 (2013), Application(s): Western blot, Abstract; Full Text
Protein kinase G oxidation is a major cause of injury during sepsis: O. Rudyk, et al.; PNAS 110, 9909 (2013), Application(s): Western blot, Abstract; Full Text
Hydrogen peroxide enhances vasodilatation by increasing dimerization of cGMP-dependent protein kinase type Iα: D. Dou, et al.; Circ. J. 76, 1792 (2012), Application(s): Western blot, Abstract;
Nitroglycerin fails to lower blood pressure in redox-dead Cys42Ser PKG1α knock-in mouse: O. Rudyk, et al.; Circulation 126, 287 (2012), Application(s): Western blot, Abstract; Full Text
cGMP-dependent protein kinase I beta physically and functionally interacts with the transcriptional regulator TFII-I: R.B. Pilz, et al. ; J. Biol. Chem. 277, 32003 (2002), Application(s): IP (M), ICC (H) using mouse samples, Abstract;
Role of myosin phosphatase isoforms in cGMP-mediated smooth muscle relaxation: S.A. Fisher, et al. ; J. Biol. Chem. 276, 37250 (2001), Application(s): IP, WB using rat samples, Abstract;

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