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PKG polyclonal antibody

 
ADI-KAP-PK002-D 50 µg 184.00 USD
 
ADI-KAP-PK002-F 200 µg 367.00 USD
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Product Specification

Alternative Name:Protein kinase G
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to a portion of human PKG1β, with the subsitiution of a tryptophan residue for tyrosine at residue 9.
 
UniProt ID:Q13976
 
GenBank ID:Y07512
 
Species reactivity:Human, Mouse, Rat
Bovine
 
Applications:ICC, IHC, IP, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~80kDa by Western blot.
 
Purity Detail:Peptide affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Cyclic GMP-dependent protein kinases (PKGs or cGKs) are classified into two types, PKGI and PKGII. Studies have shown that PKGs are highly homologous to PKAs; phosphorylation of cellular proteins by both families of kinases leads to alterations in calcium mobilization, protein phosphatase activity, ion channel function, gene transcription, smooth muscle contractility, and platelet aggregation.
 
KAP-PK002 WB
Western blot analysis of Prod. No. ADI-KAP-PK002: Lane 1: MW marker, Lane 2: HeLa cell lysate, Lane 3: Rat brain tissue extract, Lane 4: MDBK cell lysate.
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KAP-PK002 WB

Product Literature References

Alternative splicing of cGMP-dependent protein kinase I in angiotensin-hypertension: novel mechanism for nitrate tolerance in vascular smooth muscle: J.M. Simard, et al. ; Circ. Res. 93, 805 (2003), Application(s): IHC, WB using rat samples, Abstract;
Protein kinase G activation of K(ATP) channels in human-cultured prostatic stromal cells: J.M. Haynes, et al. ; Cell. Signal. 14, 1023 (2002), Application(s): ICC using human samples, Abstract;
Exisulind induction of apoptosis involves guanosine 3',5'-cyclic monophosphate phosphodiesterase inhibition, protein kinase G activation, and attenuated beta-catenin: R. Pamukcu, et al. ; Cancer Res. 60, 3338 (2000), Application(s): WB using human samples, Abstract;

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