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DNA-PK polyclonal antibody

 
ADI-KAP-PI001-E 100 µg 281.00 USD
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Product Specification

Alternative Name:DNA-dependent protein kinase
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to a portion of human DNA-PK.
 
UniProt ID:P78527
 
Gene/Protein Identifier:A57099 (PIR)
 
Species reactivity:Human, Mouse, Rat
Hamster
 
Applications:IHC (PS), WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~350kDa by Western blot.
 
Purity Detail:Immunoaffinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:DNA-dependent protein kinase (DNA-PK) is made up of 3 subunits, 2 Ku proteins, and a large catalytic subunit (DNA-PKcs). In vitro, DNA-PK phosphorylates several transcription factors and other DNA-binding proteins and is believed to act in DNA damage recognition, repair, and transcription.
 
KAP-PI001 WB
Western Blot Analysis of DNA-PK, Lane 1: MWM, Lane 2: HeLa Cell Lysate, Lane 3: Mouse Spleen Tissue Lysate, Lane 4: Rat Spleen Tissue Lysate, Lane 5: CHO-K1 Cell Lysate.
ADI-KAP-PI001 IHC1
IHC Analysis: Human prostate tissue stained with DNA-PK, pAb at 10µg/ml.
ADI-KAP-PI001 IHC2
IHC Analysis: Human testis tissue stained with DNA-PK, pAb at 10µg/ml.
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KAP-PI001 WB ADI-KAP-PI001 IHC1 ADI-KAP-PI001 IHC2

Product Literature References

ZIC2-dependent transcriptional regulation is mediated by DNA-dependent protein kinase, poly(ADP-ribose) polymerase, and RNA helicase A: A. Ishiguro, et al. ; J. Biol. Chem. 282, 9983 (2007), Application(s): WB using mouse tissue & cell lysates, Abstract;

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