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eNOS polyclonal antibody

ADI-KAP-NO023-D 50 µg 180.00 USD
ADI-KAP-NO023-F 200 µg 398.00 USD
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Product Specification

Alternative Name:NOS3, cNOS, NOS III, Nitric oxide synthase (endothelial)
Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of human eNOS.
UniProt ID:P29474
Source:Purified from rabbit serum.
Species reactivity:Human, Mouse, Rat
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~133kDa by Western blot.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:The diffusible free radical gas nitric oxide (NO) affects a variety of physiological functions, and is a key regulator of the cardiovascular, nervous, and immune systems. NO is synthesized in many tissues from L-arginine, oxygen, and NADPH by three known isoforms of a heme-containing flavoprotein termed NO synthase (NOS-I/nNOS, NOS-II/iNOS, and NOS-III/eNOS). eNOS is a constitutively expressed isoform originally characterized in vascular endothelium. eNOS is activated by the interplay of eNOS binding proteins such as calmodulin and Hsp90, as well as by posttranslational modification of the protein by phosphorylation at multiple sites including Ser1177 (activating) and Thr495 (inhibiting). eNOS activation at Ser1177 can be triggered by many protein kinases including Akt/PKB, PKA, CAMKII, or by dephosphorylation of Thr495 by phosphatases such as PP2A.
eNOS polyclonal antibody Western blot
Western blot analysis of eNOS pAb: Lane 1: MW marker, Lane 2: HeLa (heat- shocked), Lane 3: PC-12 (heat- shocked).
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eNOS polyclonal antibody Western blot

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