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eNOS polyclonal antibody

ADI-KAP-NO021-F 200 µg 360.00 USD
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Product Specification

Alternative Name:NOS3, cNOS, NOS III, Nitric oxide synthase (endothelial)
Immunogen:Synthetic peptide corresponding to a portion of human eNOS.
UniProt ID:P29474
Source:Purified from rabbit serum.
Species reactivity:Mouse, Rat
Applications:IHC (PS), WB
Recommended Dilutions/Conditions:Western Blot (1:500, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~135kDa by Western blot.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:The diffusible free radical gas nitric oxide (NO) affects a variety of physiological functions, and is a key regulator of the cardiovascular, nervous, and immune systems. NO is synthesized in many tissues from L-arginine, oxygen, and NADPH by three known isoforms of a heme-containing flavoprotein termed NO synthase (nNOS/NOS-I, iNOS/NOS-II, and eNOS/NOS-III). nNOS is a constitutively expressed neuronal NOS isoform that exits in its latent form until it is activated by the binding of calmodulin follo wing elevation of intracellular calcium levels. The C-terminus of nNOS contains a conserved serine residue, Ser1417, analogous to Ser1177 of the constitutively expressed endothelial NOS isoform (eNOS). Phosphorylation of Ser1417 is believed to regulate nNOS activation, particularly in glucose-sensing neurons, where inhibition of AMPK pathways by glucose and leptin serve to suppress nNOS activity, whereas activation of AMPK by insulin leads to nNOS activation.
eNOS polyclonal antibody Immunohistochemistry
IHC Analysis: Human spleen tissue stained with eNOS, pAb at 10µg/ml.
eNOS polyclonal antibody Western blot
Western blot analysis: Lane 1: Mouse brain, Lane 2: Rat brain; probed with eNOS pAb (Prod. No. ADI-KAP -NO021).
eNOS polyclonal antibody Immunohistochemistry
IHC Analysis: Human placenta tissue stained with eNOS, pAb at 10µg/ml.
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eNOS polyclonal antibody Immunohistochemistry eNOS polyclonal antibody Western blot eNOS polyclonal antibody Immunohistochemistry

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