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MEK1 polyclonal antibody

 
ADI-KAP-MA010-E 100 µg 281.00 USD
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Product Specification

Alternative Name:MKK1, MAP kinase kinase 1
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to the sequence near the N-terminus of human MEK1. The sequence is completely conserved in mouse, rat, hamster, rabbit and Xenopus.
 
UniProt ID:Q02750
 
GenBank ID:L05624
 
Species reactivity:Human, Mouse, Rat
Xenopus
 
Applications:IF, IHC, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:50)
Western Blot (1µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~48kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:MEK is a dual specificity kinase capable of phosphorylating both tyrosine and threonine residues. The MEK family, which is also known as MAP kinase kinase, phosphorylates MAP kinases on the conserved T-X-Y motif; phosphorylation of MAPK by MEK results in an increase in MAPK activity. MEK is involved in a diverse array of cellular processes such as stress-activated response, apoptosis, cytokine-induced cell proliferation, and DNA recombination during meiosis.
 
KAP-MA010 IF
Immunofluorescent analysis (confocal) staining of HeLa cells using MEK1 pAb (green); nuclei are stained in blue pseudocolor using DRAQ5.
KAP-MA010 IHC
Immunohistochemistry analysis of human breast cancer tissue with MEK1 pAb.
ADI-KAP-MA010 WB
Western blot analysis of MEK1, pAb (Prod. No. ADI-KAP-MA010): Lane 1: MW marker; Lane 2: HeLa (heat shocked) (Prod. No. ADI-LYT-HL-101); Lane 3: Rat spleen extract; Lane 4: Mouse brain extract (Prod. No. ADI-LYT-MB100)
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KAP-MA010 IF KAP-MA010 IHC ADI-KAP-MA010 WB

Product Literature References

Role of ether-linked lysophosphatidic acids in ovarian cancer cells: Y. Xu, et al. ; J. Lipid Res. 43, 463 (2002), Application(s): WB using human samples, Abstract;

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Brain (mouse), (tissue extract) 

Mouse brain extract., WB | Print as PDF
 
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Do you need bulk/larger quantities?
 

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