Erk1/2 polyclonal antibody



ADI-KAP-MA001-D	50 µg	216.00 USD

ADI-KAP-MA001-F	200 µg	510.00 USD
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Product Details

Alternative Name:	MAPK2/3, MAP kinase 2/3

Host:	Rabbit

Immunogen:	Synthetic peptide corresponding to a portion of rat Erk1. The sequence is completely conserved in hamster.

UniProt ID:	P21708

GenBank ID:	M61177

Source:	Purified from rabbit serum.

Species reactivity:	Human, Mouse, Rat Bovine, Chicken, Drosophila, Sheep, Xenopus

Applications:	Flow Cytometry, IHC (PS), IP, WB

Recommended Dilutions/Conditions:	Flow Cytometry (10µg/ml) Immunohistochemistry (paraffin sections, 1:50) Western Blot (1:1,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a bands of ~42kDa (Erk2) and ~44kDa (Erk1) by Western blot.

Purity Detail:	Protein A affinity purified.

Formulation:	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Scientific Background:	The Extracellular regulated kinases, Erk-1 and Erk-2, both play a critical role in cellular proliferation. These extracellular kinases, are known to reside downstream of the Mitogen activated protein kinase/Extracellular protein kinases referred to as MEK. Both MEK-1 and MEK-2 phosphorylate and activate Erk on the conserved TEY motif within the activation loop of the kinase.

Regulatory Status:	RUO - Research Use Only

Erk1/2 polyclonal antibody Immunohistochemistry

Immunohistochemistry analysis: Human placenta tissue stained with Erk1/2, pAb at 10µg/ml.

Erk1/2 polyclonal antibody Flow Cytometry

Flow cytometry analysis of U251 cells using Erk1/2 pAb (right) and control rabbit IgG (left).

Western blot analysis of human A431 fibroblast cell lysate, probed with Erk1/2 pAb.

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Product Literature References

A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;

EGF-mediated EGFR/ERK signaling pathway promotes germinative cell proliferation in Echinococcus multilocularis that contributes to larval growth and development: Z. Cheng, et al.; PLoS Negl. Trop. Dis. 11, e0005418 (2017), Abstract; Full Text

Cognitive impairments following cranial irradiation can be mitigated by treatment with a tropomyosin receptor kinase B agonist: P. Yang, et al.; Exp. Neurol. 279, 178 (2016), Application(s): Immunoblotting, Abstract;

Dendritic Cell Differentiation Induced by a Self-Peptide Derived from Apolipoprotein E: B. Singh, et al. ; J. Immunol. 181, 6859 (2008), Application(s): WB using mouse cell lysates, Abstract;

Reduction of chronic allograft nephropathy by inhibition of extracellular signal-regulated kinase 1 and 2 signaling: C. Du, et al. ; Am. J. Physiol. Renal Physiol. 295, F672 (2008), Application(s): WB, IHC using mouse tissue, Abstract;

Surface cytotoxic T lymphocyte-associated antigen 4 partitions within lipid rafts and relocates to the immunological synapse under conditions of inhibition of T cell activation: J. Madrenas, et al. ; J. Exp. Med. 195, 1337 (2002), Application(s): WB using human samples, Abstract;

Stress-induced inhibition of ERK1 and ERK2 by direct interaction with p38 MAP kinase: S. Pelech, et al. ; J. Biol. Chem. 276, 6905 (2001), Application(s): IP using human samples, Abstract;

Zap-70-independent Ca(2+) mobilization and Erk activation in Jurkat T cells in response to T-cell antigen receptor ligation: R.L. Wange, et al. ; Mol. Cell. Biol. 21, 7137 (2001), Application(s): WB using human samples, Abstract;

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