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RAB4 polyclonal antibody

ADI-KAP-GP005-F 200 µg 352.00 USD
ADI-KAP-GP005-J 1 mg 1,382.00 USD
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Product Specification

Alternative Name:Ras-related protein Rab-4
Immunogen:Recombinant human Rab4.
UniProt ID:P20338
Species reactivity:Mouse, Rat
Crossreactivity:Weakly cross-reacts with human RAB4.
Applications:ICC, IHC (PS), WB
Recommended Dilutions/Conditions:Western Blot (1:500)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~25kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:The Rab family of GTP-binding proteins are members of the Ras superfamily of small G proteins, and participate in multiple steps of the membrane trafficking. Rab3A participates in the fusion of synaptic vesicles during neurotransmitter release, a process regulated by GDP/GDP exchange cofactors such as GAP, GDI, GRF, and rabphilin. Rab4 regulates recycling of proteins from the early endosome to the surface of many cell types, regulating glucose transporter and transferrin receptor cell surface localization. Rab5 regulates the fusion of plasma membrane-derived clathrin-coated vesicles with early endosomes and homotypic fusion among early endosomes.
Western blot analysis of Rab4: Lane 1: MW marker, Lane 2: HeLa Cell Lysate (Heat Shocked), Lane 3: Rat Brain Tissue Extract, Lane 4: Mouse Brain Tissue Extract.
Immunohistochemistry analysis of human pancreas tissue stained with RAB4 pAb at 15µg/ml.
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Product Literature References

An evaluation of the expression, subcellular localization, and function of rab4 in the exocrine pancreas: J.D. Jamieson, et al. ; Biochem. Biophys. Res. Commun. 268, 847 (2000), Application(s): WB using human samples, Abstract;
The small GTP-binding protein rab4 is associated with early endosomes: P. van der Sluijs, et al.; PNAS 88, 6313 (1991), Application(s): ICC using human samples, Abstract;

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