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[pThr210]Plk polyclonal antibody

 
ADI-KAP-CC107-D 50 µg 205.00 USD
 
ADI-KAP-CC107-F 200 µg 399.00 USD
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Product Specification

Alternative Name:Polo-like kinase
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to a portion of mouse PLK1 phosphorylated at Thr210.
 
UniProt ID:Q07832
 
Gene/Protein Identifier:NP_035251 (RefSeq)
 
Species reactivity:Human
 
Crossreactivity:Weakly cross-reacts with mouse, rat and Drosophila Plk.
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~68kDa by Western blot.
 
Purity Detail:Immunoaffinity purified.
 
Formulation:Liquid. In TRIS-glycine, pH 7.0, containing 43% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The polo-like kinases (PLKs) function in cell-cycle progression and in multiple stages of mitosis. PLK1 is activated by phosphorylation of Thr210 at the G2/M phase boundary. Active PLK1 participates in mitotic entry through activation of Cdc25C and nuclear import of cyclin B1, thereby activating the Cdc2/cyclin B kinase.
 

Product Literature References

Cyclin F suppresses B-Myb activity to promote cell cycle checkpoint control: D. K. Klein, et al.; Nat. Commun. 6, 5800 (2015), Application(s): Western Blotting, Abstract;

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