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p70 S6K polyclonal antibody

ADI-KAP-CC035-E 100 µg 347.00 USD
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Product Specification

Alternative Name:ribosomal protein S6 kinase
Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of human p70 S6K.
UniProt ID:P23443 (S6K1), Q9UBS0 (S6K2)
Species reactivity:Human, Mouse, Rat
Applications:ELISA, IP, WB
Recommended Dilutions/Conditions:ELISA (1µg/ml)
Immunoprecipitation (6 µg/500-1500µg protein)
Western Blot (1µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~70kDa by Western blot.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In TBS containing 0.05% sodium azide.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:p70 S6K is a mitogen stimulated serine/threonine protein kinase that regulates the cell growth and G1 phase cell cycle progression. p70 S6K is activated by serum stimulation and can be inhibited by both wortmannin and rapamycin. Activation of p70 S6K by multi-site phosphorylation requires phosphoinositide 3-kinase (PI3-K)-dependent signal(s) leading to sequential phosphorylation at two key residues: Thr389 and Thr229. Thr229 phosphorylation is mediated by phosphoinositide-dependent kinase 1 (PDK-1), and is dependent upon prior phosphorylation of Thr389. Multiple residues in the p70 S6K pseudosubstrate domain (e.g. Ser411, Thr421, and Ser424) are also subject to activating phosphorylation by upstream kinases.
Western blot analysis of p70 S6K immunoprecipitated from mouse brain extract and probed with p70 S6K pAb. 1: Sample immunoprecipitated with an irrelevant antibody (p90 RSK1 pAb) as a negative control. 2: Sample immunoprecipitated with p70 S6K pAb.
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Product Literature References

Lysosomal calcium signalling regulates autophagy through calcineurin and TFEB: D.L. Medina, et al.; Nat. Cell. Biol. 17, 288 (2015), Application(s): Western Blotting, Abstract;

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