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[pSer28]Histone H3 polyclonal antibody

 
ADI-KAP-CC012-D 50 µg 301.00 USD
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Product Specification

Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to a portion of human Histone H3 phosphorylated at Ser28.
 
UniProt ID:P68431 (Histone H3.1), Q71DI3 (Histone H3.2), P84243 (Histone H3.2)
 
Species reactivity:Human
 
Applications:ICC, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:100-1:1,000)
Western Blot (1:200-1:2,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~15-17kDa by Western blot.
 
Purity Detail:Peptide affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The core histones form an octamer, which is made up of one H3-H4 tetramer and two H2A-H2B dimers. The N-terminal tail of core histones undergoes different post-transcriptional modifications including acetylation, phosphorylation, methylation and ubiquitination. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.
 
KAP-CC012 WB
Western blot analysis of M-phase arrested HeLa using Histone H3 (pSer28) pAb
KAP-CC012 ICC
Immunocytochemistry analysis of Histone H3 (pSer28) on acetone fixed Hep-2.
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KAP-CC012 WB KAP-CC012 ICC

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