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Phosphotyrosine monoclonal antibody (6D12)

 
ADI-KAM-TK150-F 200 µg 301.00 USD
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Product Specification

Clone:6D12
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Phosphotyrosine conjugated to KLH.
 
Species reactivity:Human
 
Applications:ICC, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (10-50µg/ml)
Western Blot (1-10µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Protein phosphorylation is a complex biochemical process that is regulated by protein kinases that catalyze the transfer of the gamma-P of ATP to a recipient protein that acts as a substrate. This post-translational modification is primarily directed onto serine, threonine, and tyrosine amino acid residues within a protein. Changes in the phosphorylation status of phosphoproteins can alter their biological function, and aberrations in signal transduction cascades have been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders.
 
KAM-TK150 WB
Western blot analysis of human A431 cells unstimulated (1) or EGF-stimulated (2) probed with Phosphotyrosine mAb (6D12).
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KAM-TK150 WB

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