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[pSer32/Ser36]IκBα monoclonal antibody (39A1413)

 
ADI-KAM-TF140-E 100 µg 313.00 USD
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Replaces Prod. #: ALX-804-235

Product Specification

Clone:39A1413
 
Host:Mouse
 
Isotype:IgG1κ
 
Immunogen:Synthetic peptide corresponding to a portion of human IκBα phosphorylated at Ser32/36.
 
UniProt ID:P25963
 
Species reactivity:Human, Mouse, Rat
Bovine, Dog, Porcine
 
Applications:IP, WB
 
Recommended Dilutions/Conditions:Immunoprecipitation (1-2µg/ml)
Western Blot (1-2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~42kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:NF-κB is silenced in the cytoplasm by the inhibitory protein IkappaBa, which masks nuclear localization signals on NF-κB. IkappaBa is phosphorylated by the IkappaB kinase (IKK) complex in response to external stimuli such as tumor necrosis factor or other cytokines. IKK specifically phosphorylates IkappaBa on Ser32 and Ser36, leading to ubiquitination of IkappaBa and subsequent degradation by the 26S proteasome. Degradation of IkappaBa exposes the nuclear localization signal of NF-κB dimers, which then translocate to the nucleus to activate target genes
 
KAM-TF140 WB
Western blot analysis of Jurkat cells treated for 30 min with ALLN, a calpain and proteasome inhibitor that prevents IkBalpha dephosphorylation, followed by incubation without (1) or with (2) TNF-alpha, probed with IkBalpha mAb (pSer32/36) (39A1413).
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KAM-TF140 WB

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