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GSK-3α/β monoclonal antibody (1H8)

 
ADI-KAM-ST002-D 50 µg 194.00 USD
 
ADI-KAM-ST002-F 200 µg 367.00 USD
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Replaces Prod. #: ALX-804-178

Product Specification

Alternative Name:Glycogen synthase kinase-3α/β
 
Clone:1H8
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:Recombinant Xenopus GSK-3β.
 
UniProt ID:Q91757
 
Species reactivity:Human, Mouse, Rat
Bovine, Dog, Hamster, Monkey, Porcine, Rabbit, Sheep
 
Applications:ICC, IHC, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~51 and ~47kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Glycogen Synthase Kinase 3β (GSK-3β) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3β on serine 9, which prevents GSK-3β from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3β is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3β. The phosphorylated serine binds to the GSK-3β priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3β participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3β is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3β via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3β with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3β inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.
 
KAM-ST002 WB
Western blot analysis: Lane 1: MW marker, Lane 2: 20µg HeLa lysate (heat shocked), Lane 3: 20µg mouse brain lysate, Lane 4: 20µg rat brain lysate, Lane 5: 20µg RK-13 lysate.
Please mouse over
KAM-ST002 WB

Product Literature References

Intracellular targets of paullones: identification following affinity purification on immobilized inhibitor: L. Meijer, et al. ; J. Biol. Chem. 277, 25493 (2002), Application(s): WB using porcine & rat samples, Abstract;

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