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β-Catenin monoclonal antibody (12F7)

 
ADI-KAM-ST001-E 100 µg 258.00 USD
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Product Specification

Alternative Name:CTNNB
 
Clone:12F7
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant β-catenin (N-terminal fragment).
 
UniProt ID:P35222 (human), Q02248 (mouse), Q9WU82 (rat)
 
Species reactivity:Human, Mouse, Rat
 
Applications:IHC, IP, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1-5µg/ml)
Immunoprecipitation (2µg/200-300µl cell extract)
Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~97kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:β-Catenin, a vertebrate homologue of Drosophila armadillo encoded by the CTNNB1 gene, performs two functions in the cell: mediating cell-cell adhesion by association between E-cadherin and the cytoskeleton, and acting as a downstream transcriptional activator of Wnt signaling, a key player in such functions as embryonic development, the induction of cell polarity, and the determination of cell fate.  In the absence of Wnt signaling, glycogen synthase kinase-3β (GSK-3β) phosphorylates any free β-catenin at the amino terminus.  This results in adenomatous polyposis coli tumor suppressor protein (APC)-mediated β-catenin degradation via the ubiquitin-proteosome pathway.  Wnt signaling stabilizes β-catenin by inhibiting the activity of GSK-3β and preventing β-catenin degradation.  β-catenin forms complexes with DNA-binding proteins such as T-cell factor (Tcf) and lymphocyte-enhancer factor (Lef), which promote  expression of a variety of target genes, including c-myc and cyclin D1.
 
KAM-ST001 WB
Western blot analysis of A431 cells (1), Hela cells (2), NIH/3T3 cells (3), C2C12 cells (4) and Rat-1 cells (5) probed with β-catenin mAb (12F7)
KAM-ST001 IP
Immunoprecipitation of Rat-1 cells with normal mouse IgG (1) or β-catenin mAb (12F7 (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with β-catenin mAb (12F7).
KAM-ST001 IHC1
Immunohistochemical detection of gastric epithelium β-catenin on paraffin embedded sections with β-Catenin mAb (12F7).
KAM-ST001 IHC2
Immunohistochemical detection of colorectal cancer β-catenin on paraffin embedded sections with β-catenin mAb (12F7).
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KAM-ST001 WB KAM-ST001 IP KAM-ST001 IHC1 KAM-ST001 IHC2

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