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PKCα monoclonal antibody (MC5)

 
ADI-KAM-PK020-D 50 µg 184.00 USD
 
ADI-KAM-PK020-F 200 µg 378.00 USD
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Replaces Prod. #: BML-SA143

Product Specification

Alternative Name:Protein kinase Cα
 
Clone:MC5
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Bovine brain PKCα.
 
UniProt ID:P04409
 
Species reactivity:Human, Mouse, Rat
Bovine, Dog, Fish, Gerbil, Monkey, Porcine, Rabbit, Sheep
 
Applications:Flow Cytometry, IF, IHC, IP, WB
 
Recommended Dilutions/Conditions:Flow Cytometry (10µg/ml)
Immunohistochemistry (1:50, human lung, carcinoma tissue)
Immunoprecipitation (12.5µg/ml)
Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~80kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Protein Kinase C (PKC) is a large superfamily of serine/threonine kinases that mediate essential cellular signals required for activation, proliferation, differentiation and survival. There are at least ten PKC isotypes that are closely related in structure but that have distinct patterns of tissue distribution and function. The PKC isotypes can be subdivided into three classes based on primary structure and biochemical properties. These are: classical or conventional PKC isotypes (cPKC), novel PKC isotypes (nPKC) and atypical PKC isotypes (aPKC).
 
KAM-PK020 WB
Western blot analysis: Lane 1 : MW marker, Lane 2: HeLa cell lysate, Lane 3: mouse brain tissue extract, Lane 4: rat brain tissue extract, Lane 5: 3T3 cell lysate, probed with PKCα, mAb (MC5).
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KAM-PK020 WB

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