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Nucleolin monoclonal antibody (4E2)

 
ADI-KAM-CP100-E 100 µg 323.00 USD
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Product Specification

Clone:4E2
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Human nucleolin from Raji cell extract.
 
UniProt ID:P19338
 
Species reactivity:Human
 
Applications:Flow Cytometry, ICC, IHC, WB
 
Recommended Dilutions/Conditions:Flow Cytometry (5-10µg/ml final concentration)
Immunocytochemistry (1-10µg/ml)
Immunohistochemistry (1-10µg/ml)
Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~100kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Nucleolin (identical to human DNA helicase IV) is an abundant, non-ribosomal protein of the nucleolus involved in the organization of nuclear chromatin, rDNA transcription, packaging of pre-RNA, ribosome assembly, nucleocytoplasmic transport, cytokinesis, nucleogenesis, and apoptosis. A predominantly nuclear protein, the presence of nucleolin at the surface of cancer cells suggests that it could be valuable as a marker for the diagnosis of cancer.
 
KAM-CP100 WB
Western blot analysis of HL60 (1) and ZR-75-1 (2) cell extracts, probed with Nucleolin mAb (4E2).
KAM-CP100 IHC
Immunohistochemistry analysis of nuclear staining of colon cancer sections using Nucleolin mAb (4E2).
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KAM-CP100 WB KAM-CP100 IHC

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