Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Nucleolin monoclonal antibody (4E2)

 
ADI-KAM-CP100-E 100 µg 339.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Clone:4E2
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Human nucleolin from Raji cell extract.
 
UniProt ID:P19338
 
Species reactivity:Human
 
Applications:Flow Cytometry, ICC, IHC, WB
 
Recommended Dilutions/Conditions:Flow Cytometry (5-10µg/ml final concentration)
Immunocytochemistry (1-10µg/ml)
Immunohistochemistry (1-10µg/ml)
Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~100kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Nucleolin (identical to human DNA helicase IV) is an abundant, non-ribosomal protein of the nucleolus involved in the organization of nuclear chromatin, rDNA transcription, packaging of pre-RNA, ribosome assembly, nucleocytoplasmic transport, cytokinesis, nucleogenesis, and apoptosis. A predominantly nuclear protein, the presence of nucleolin at the surface of cancer cells suggests that it could be valuable as a marker for the diagnosis of cancer.
 
Nucleolin monoclonal antibody (4E2) Western blot
Western blot analysis of HL60 (1) and ZR-75-1 (2) cell extracts, probed with Nucleolin mAb (4E2).
Nucleolin monoclonal antibody (4E2) Immunohistochemistry
Immunohistochemistry analysis of nuclear staining of colon cancer sections using Nucleolin mAb (4E2).
Please mouse over
Nucleolin monoclonal antibody (4E2) Western blot Nucleolin monoclonal antibody (4E2) Immunohistochemistry

Product Literature References

A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;
Centrosomal nucleolin is required for microtubule network organization: X. Gaume, et al.; Cell Cycle 14, 902 (2015), Abstract; Full Text
Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA: M. Schertzer, et al.; Nucleic Acids Res. 43, 1834 (2015), Abstract; Full Text

Related Products

Goat anti-mouse IgG F(ab')2, polyclonal antibody (HRP conjugate) 

ELISA, IHC, WB | Print as PDF
 
ADI-SAB-100-J 1 ml 124.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
Nucleolin
By biological activity:
Nucleolin Monoclonal antibody
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2019 Enzo Life Sciences, Inc.,