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[pSer55]Vimentin monoclonal antibody (4A4)

 
ADI-KAM-CC249-E 100 µg 313.00 USD
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Product Specification

Alternative Name:VIM
 
Clone:4A4
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:Synthetic peptide corresponding to a portion of mouse vimentin phosphorylated at Ser55.
 
UniProt ID:P20152
 
Species reactivity:Human, Mouse, Rat
 
Applications:ELISA, ICC, WB
 
Recommended Dilutions/Conditions:ELISA, Immunocytochemistry (1µg/ml)
Western Blot (1-5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~57kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Vimentin is one of four type II intermediate filament (IF) proteins; Desmin, Glial fibrillary acidic protein (GFAP) and Peripherin being the others. Vimentin is the most widely distributed of all IF proteins. It is typically expressed in leukocytes, blood vessel endothelial cells, and mesenchymal cells such as fibroblasts. Vimentin filaments help support cellular membranes. Vimentin networks also may help keep the nucleus and other organelles in a defined place within the cell.
 
KAM-CC249 WB
Western blot analysis of U251 interphase (1) and U251 M phase (2) cells, probed with Vimentin (pSer55) mAb (4A4).
KAM-CC249 ICC
Immunocytochemistry analysis of U251 cells using Vimentin (pSer55) mAb (4A4).
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KAM-CC249 WB KAM-CC249 ICC

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