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[pSer38]Vimentin monoclonal antibody (TM38)

 
ADI-KAM-CC247-E 100 µg 301.00 USD
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Product Specification

Alternative Name:VIM
 
Clone:TM38
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Synthetic peptide corresponding to a portion of human Vimentin phosphorylated at Ser38.
 
UniProt ID:P08670
 
Species reactivity:Human
 
Applications:ICC, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (5-10µg/ml for chemiluminescence detection system)
Western Blot (3-5µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~57kDa by Western blot.
 
Formulation:Liquid. In PBS containing 50% glycerol. No preservatives.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Vimentin is one of four type II intermediate filament (IF) proteins; Desmin, Glial fibrillary acidic protein (GFAP) and Peripherin being the others. Vimentin is the most widely distributed of all IF proteins. It is typically expressed in leukocytes, blood vessel endothelial cells, and mesenchymal cells such as fibroblasts. Vimentin filaments help support cellular membranes. Vimentin networks also may help keep the nucleus and other organelles in a defined place within the cell.
 
KAM-CC247 WB
Western blot analysis of phosphorylated vimentin (Ser38) expression in U251 using D094-3.
KAM-CC247 ICC
Immunocytochemistry analysis of phosphorylated vimentin (pSer38) on 4% PFA fixed U251 cells with Prod. No. ADI-KAM-CC247.
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KAM-CC247 WB KAM-CC247 ICC

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