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[pThr1342]DNA-topoisomerase II α monoclonal antibody (3D4)

 
ADI-KAM-CC220-E 100 µg 281.00 USD
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Product Specification

Clone:3D4
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to a portion of human Topoisomerase IIα phosphorylated at Tyr1342.
 
UniProt ID:P11388 (α isozyme), Q02880 (β isozyme)
 
Species reactivity:Human
 
Applications:ICC, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (10µg/ml)
Western Blot (1-10µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~170kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:DNA-topoisomerase II, also known as Topo II or Top 2, is a multifunctional nuclear enzyme required during DNA replication, chromosome disjunction at mitosis, and other DNA-related activities because of its ability to alter DNA supercoiling. In mammalian cells, Topo II consists of two isozymes, Topo II-alpha (170kDa) and Topo II-beta (180kDa) and expression and localization of each isoform are distinct and stage specific during the cell cycle. Topo II-beta is expressed constitutively throughout the cell cycle, whereas the expression of Topo II-alpha is cell cycle-regulated, peaking in G2 to M phase and declining to a minimal level at the end of M phase. DNA-topoisomerase II is the target of some of the most active drugs used in chemotherapy and has been shown to have a critical role in neural development.
 
KAM-CC220 WB
Western blot analysis of Jurkat cells using DNA-Topoisomerase IIa (pThr1342) mAb (3D4).
KAM-CC220 ICC
Immunocytochemistry analysis of human HEp-2 cells stained with DNA-Topoisomerase IIa (pThr1342) mAb (3D4).
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KAM-CC220 WB KAM-CC220 ICC

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