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Cyclin D1 monoclonal antibody (5D4)

 
ADI-KAM-CC200-E 100 µg 282.00 USD
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Product Specification

Alternative Name:CCND1
 
Clone:5D4
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Recombinant human cyclin D1.
 
UniProt ID:P24385
 
Species reactivity:Human, Mouse
 
Applications:Flow Cytometry, ICC, IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Flow Cytometry (10µg/ml final concentration)
Immunohistochemistry (10µg/ml, frozen/paraffin)
Immunoprecipitation (1-10µg/200µg cell extract from 5x106 cells)
Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~36kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Cyclins are a family of proteins which function as the regulatory subunits of cyclin/cyclin-dependent kinase (Cdk) holoenzymes, mediating entry into and progression through the cell cycle. Each cyclin is expressed in a temporal fashion during the cell cycle, partnering with a specific Cdk to activate its protein kinase activity. The most well defined mitotic cyclins are: cyclin D, which partners with cdks 4 and 6 during early G1 phase; cyclin E, which activates Cdk2 in the G1 to S phase transition; cyclin A, which utilizes Cdks 1 and 2 during progression through S phase and into G2; and cyclin B, which activates Cdk1 in M-phase promotion.
 
Cyclin D1 monoclonal antibody (5D4) Western blot
Western blot analysis of ZR- 75-1 (1), MCF7 (2), HeLa (3), WR19L (4) and NIH/3T3 (5) cells probed with Cyclin D1 mAb (5D4).
Cyclin D1 monoclonal antibody (5D4) Immunoprecipitation
Immunoprecipitation analysis of cyclin D1 from ZR75-1 cells with Mouse IgG2a (1) or Cyclin D1 mAb (5D4) (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoBlotted with 553.
Cyclin D1 monoclonal antibody (5D4) Flow Cytometry
Flow cytometry analysis of Cyclin D1 expression in HeLa cells. Open histogram indicates the reaction of Isotypic control to the cells. Shaded histogram indicates the reaction of Cyclin D1 mAb (5D4) to the cells.
Cyclin D1 monoclonal antibody (5D4) Immunohistochemistry
Immunohistochemistry analysis of Cyclin D1 on paraffin embedded section of human mantle cell lymphoma with Cyclin D1 mAb (5D4).
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Cyclin D1 monoclonal antibody (5D4) Western blot Cyclin D1 monoclonal antibody (5D4) Immunoprecipitation Cyclin D1 monoclonal antibody (5D4) Flow Cytometry Cyclin D1 monoclonal antibody (5D4) Immunohistochemistry

Product Literature References

A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;

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