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Chk2 monoclonal antibody (DCS-273)

 
ADI-KAM-CC113-E 100 µg 242.00 USD
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Product Specification

Alternative Name:Checkpoint kinase 2
 
Clone:DCS-273
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Recombinant human Chk2.
 
UniProt ID:O96017
 
Species reactivity:Human
 
Applications:IHC, IP, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1-5µg/ml)
Immunoprecipitation (3µg/200-300µl, cell extract)
Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The DNA damage checkpoint is a signal transduction pathway that has the ability to delay entry into mitosis. When DNA is damaged, the Check point kinases (CHKs) are known to act downstream of ATM to elicit appropriate responses such as cell cycle arrest. Chk1, for instance, is activated by ATM phosphorylation of serines 123, 178, 278, and 292 of the S phase-promoting cdc25A phosphatase and accelerates IR (ionizing radiation)induced degradation of cdc25A.
 
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