Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Plk1 monoclonal antibody (3F8)

 
ADI-KAM-CC105-D 50 µg 184.00 USD
 
ADI-KAM-CC105-F 200 µg 378.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:Polo-like kinase 1
 
Clone:3F8
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant fragment from the middle of human PLK1.
 
UniProt ID:P53350
 
Species reactivity:Human, Rat
 
Crossreactivity:Weakly cross-reacts with mouse Plk1.
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~62kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The polo-like kinases (PLKs) function in cell-cycle progression and in multiple stages of mitosis. PLK1 is activated by phosphorylation of Thr210 at the G2/M phase boundary. Active PLK1 participates in mitotic entry through activation of Cdc25C and nuclear import of cyclin B1, thereby activating the Cdc2/cyclin B kinase.
 
KAM-CC105 WB
Western blot analysis of PLK1 mAb (3F8): Lane 1: MW marker, Lane 2: HeLa, Lane 3: Jurkat, Lane 4: PC-12, Lane 5: 3T3, Lane 6: HEK-293.
Please mouse over
KAM-CC105 WB

Product Literature References

Skp1-Cul1-F-box ubiquitin ligase (SCF(βTrCP))-mediated destruction of the ubiquitin-specific protease USP37 during G2-phase promotes mitotic entry: A.C. Burrows, et al.; J. Biol. Chem. 287, 39021 (2012), Application(s): Western blot, Abstract; Full Text

Related Products

Jurkat, (cell lysate) 

Human, WB | Print as PDF
 
ADI-LYC-JK100-F 200 µg 109.00 USD
Do you need bulk/larger quantities?
 

PC-12, (cell lysate) 

Rat, WB | Print as PDF
 
ADI-LYC-PC100-F 200 µg 109.00 USD
Do you need bulk/larger quantities?
 

3T3, (cell lysate) 

Mouse, WB | Print as PDF
 
ADI-LYC-3T100-F 200 µg 103.00 USD
Do you need bulk/larger quantities?
 

Goat anti-mouse IgG, polyclonal antibody (AP conjugate) 

ELISA, WB | Print as PDF
 
ADI-SAB-101-J 1 ml 129.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
Plk
By biological activity:
Plk Monoclonal antibody
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,