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Cdk1/cdc2 monoclonal antibody (5F6)

 
ADI-KAM-CC101-E 100 µg 291.00 USD
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Product Specification

Alternative Name:Cyclin-dependent kinase 1, p34cdc2, Cell division cycle 2
 
Clone:5F6
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to the sequence near the N-terminus of human Cdk1 (cdc2).
 
UniProt ID:P06493
 
Species reactivity:Human, Mouse, Rat
Rabbit
 
Applications:IHC, IP, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (5µg/ml)
Immunoprecipitation (2µg/200µl)
Western Blot (1-5µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~34kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The cell division cycle (cdc) group of proteins consists of many protein classes, including cyclin dependent kinases (Cdks), phosphoprotein phosphatases, mitogen activated kinases, small GTPases, and cyclins. These proteins play a central role in regulating progression through the cell cycle through the formation and/or modification of key substrates. Cdc42 belongs to the Rho-family of GTPases, and promotes actin polymerization to form filopodia. Cdc25A is a dual-specificity phosphatase that acts on phosphorylated threonine and tyrosine residues of Cdks, leading to binding and activation of their partner cyclins.
 
KAM-CC101 WB
Western blot analysis of human HeLa cell lysate probed with Cdk1/cdc2 mAb (5F6).
KAM-CC101 IP
Immunoprecipitation analysis of Cdk1/cdc2 from A431 cells extract with normal mouse IgG (1) or Cdk1/cdc2 mAb (5F6) (2). After immunoprecipitation with the antibody, the immunocomplex was resolved by SDS-PAGE and immunoBlotted with Cdk1/cdc2 mAb (5F6). A431 crude lysate was resolved in lane 3.
KAM-CC101 IHC
Immunohistochemistry analysis of paraffin embedded section of rat testis using Cdk1/cdc2 mAb (5F6).
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KAM-CC101 WB KAM-CC101 IP KAM-CC101 IHC

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