| ADI-KAM-CC086-E | 100 µg | 271.00 USD |  |
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| Alternative Name: | Cell division cycle 25A |
| Clone: | DCS-121 |
| Host: | Mouse |
| Isotype: | IgG2a |
| Immunogen: | Recombinant human cdc25A. |
| UniProt ID: | P30304 |
| GenBank ID: | AY137580 |
| Gene/Protein Identifier: | NM_001789 (RefSeq) |
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC, WB |
| Recommended Dilutions/Conditions: | Immunohistochemistry (1-5µg/ml) Immunprecipitation (3µg/200-300µl, cell extract) Western Blot (1-5µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Application Notes: | Detects bands of ~67kDa and ~60kDa by Western blot. |
| Purity Detail: | Protein A affinity purified. |
| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol. |
| Shipping: | Shipped on Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | The cell division cycle (cdc) group of proteins consists of many protein classes, including cyclin dependent kinases (Cdks), phosphoprotein phosphatases, mitogen activated kinases, small GTPases, and cyclins. These proteins play a central role in regulating progression through the cell cycle through the formation and/or modification of key substrates. Cdc42 belongs to the Rho-family of GTPases, and promotes actin polymerization to form filopodia. Cdc25A is a dual-specificity phosphatase that acts on phosphorylated threonine and tyrosine residues of Cdks, leading to binding and activation of their partner cyclins. |
