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Cdc25A monoclonal antibody (DCS-121)

 
ADI-KAM-CC086-E 100 µg 258.00 USD
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Product Specification

Alternative Name:Cell division cycle 25A
 
Clone:DCS-121
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Recombinant human cdc25A.
 
UniProt ID:P30304
 
GenBank ID:AY137580
 
Gene/Protein Identifier:NM_001789 (RefSeq)
 
Species reactivity:Human, Mouse, Rat
 
Applications:IHC, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1-5µg/ml)
Immunprecipitation (3µg/200-300µl, cell extract)
Western Blot (1-5µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~67kDa and ~60kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The cell division cycle (cdc) group of proteins consists of many protein classes, including cyclin dependent kinases (Cdks), phosphoprotein phosphatases, mitogen activated kinases, small GTPases, and cyclins. These proteins play a central role in regulating progression through the cell cycle through the formation and/or modification of key substrates. Cdc42 belongs to the Rho-family of GTPases, and promotes actin polymerization to form filopodia. Cdc25A is a dual-specificity phosphatase that acts on phosphorylated threonine and tyrosine residues of Cdks, leading to binding and activation of their partner cyclins.
 
KAM-CC086 WB
Western blot analysis of human HeLa cell lysate (1), mouse NIH/3T3 cell lysate (2) and rat Rat-1 cell lysate (3), probed with cdc25A mAb (DCS -121)
KAM-CC086 IHC
Immunohistochemistry analysis of paraffin-embedded human tonsil (germinal center) stained with cdc25A mAb (DCS-121).
KAM-CC086 IP
Immunoprecipitation analysis of cdc25A from Raji cells with normal mouse IgG (1) or cdc25A mAb (DCS-121) (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoBlotted with cdc25A mAb (DCS -121).
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KAM-CC086 WB KAM-CC086 IHC KAM-CC086 IP

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