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p53 monoclonal antibody (PAb122)

ADI-KAM-CC002-D 50 µg 184.00 USD
ADI-KAM-CC002-F 200 µg 389.00 USD
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Product Specification

Alternative Name:TRP53, Tumor protein p53
Immunogen:SV40 transformed 3T3 cells.
UniProt ID:P02340
Species reactivity:Human, Mouse, Rat
Crossreactivity:Weakly cross-reacts with dog p53.
Applications:Flow Cytometry, ICC, IHC, IP, WB
Recommended Dilutions/Conditions:Western Blot (1:250 colorimetric, 1:250 ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~48/53kDa by Western blot.
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to Mdm2, SV40 T antigen and human Papilloma virus E6 protein. p53 senses DNA damage and facilitates its repair. Mutation involving p53 is found in a wide variety of malignant tumors, including breast, ovary, bladder, colon, lung, and melanoma.
Western blot analysis of p53: Lane 1: MW marker, Lane 2: A431, Lane 3: rat brain, Lane 4: mouse brain.
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Product Literature References

Pulmonary tumors associated with the JC virus T-antigen in a transgenic mouse model: A. Noguchi, et al.; Oncol. Rep. 30, 2603 (2013), Application(s): Immunohistochemistry of mouse tissue, Abstract; Full Text
Abdominal temperature induces region-specific p53-independent apoptosis in the cauda epididymidis of the mouse: M. Jara, et al.; Biol. Reprod. 67, 1189 (2002), Application(s): WB using mouse tissue homogenates, Abstract; Full Text

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