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HSP27 (low endotoxin) (human), (recombinant)

 
ADI-ESP-715-D 50 µg 344.00 USD
 
ADI-ESP-715-F 200 µg 792.00 USD
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Product Specification

Alternative Name:HspB1, Heat shock protein 27
 
Recommended Dilutions/Conditions:Western Blot (100ng, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
MW:~27kDa
 
Source:Produced in E. coli.
 
UniProt ID:P04792
 
GenBank ID:L39370
 
Formulation:Liquid. In Dulbecco's PBS.
 
Purity:≥90% (SDS-PAGE; Western blot)
 
Purity Detail:Purified by multi-step chromatography.
 
Endotoxin Content:<50EU/mg purified protein (LAL test)
 
Applications:WB
 
Application Notes:Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha, p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.
 
ADI-ESP-715 SDS-PAGE
Figure 1: SDS-PAGE Analysis - Lane 1: MW Marker; Lane 2: 1μg Hsp27
ADI-ESP-715 WB
Figure 2: Western Blot Analysis – Lane 1: MW Marker; Lane 2: 100ng Hsp27; Lane 3: 20μg HeLa lysate; Lane 4: 20μg Heat-shocked HeLa lysate.
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ADI-ESP-715 SDS-PAGE ADI-ESP-715 WB

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