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HSP70 (low endotoxin) (human), (recombinant)

 
ADI-ESP-555-D 50 µg 317.00 USD
 
ADI-ESP-555-F 200 µg 792.00 USD
 
Replaces Prod. #: ALX-201-214

Product Specification

Alternative Name:Heat shock protein 70, HspA1A, HspA1B, HSP72
 
Recommended Dilutions/Conditions:Western Blot (100ng, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
MW:~72kDa
 
Source:Produced in E. coli.
 
UniProt ID:P08107
 
GenBank ID:M11717
 
Gene/Protein Identifier:NP_005336.3 (RefSeq), NM_005345 (RefSeq), 3303 (Entrez GeneID), 140550 (OMIM)
 
Formulation:Liquid. In Dulbecco’s PBS.
 
Purity:≥90% (SDS-PAGE; Western blot)
 
Purity Detail:Purified by multi-step chromatography.
 
Endotoxin Content:<50EU/mg purified protein (LAL test)
 
Applications:ELISA, WB
Activity assay, in vitro Assay
 
Application Notes:ATPase activity assay (positive). Western blot control.
 
Long Term Storage:-80°C
 
Miscellaneous/General:The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
 
ADI-ESP-555 SDS-PAGE
SDS-PAGE analysis of HSP70 (low endotoxin) (human), (recombinant): Lane 1: MW marker, Lane 2: 1µg, Lane 3: 2µg, Lane 4: 5µg HSP70.
ADI-ESP-555 WB
Western Blot Analysis of HSP70 (low endotoxin) (human), (recombinant): Left blot probed with HSP70 mAb (Prod. No. ADI-SPA-810) - Lane 1: MW Marker, Lane 2: HeLa lysate (Prod. No. ADI-LYC-HL100), Lane 3: Heat-shocked HeLa lysate (Prod. No. ADI-LYC-HL101), Lane 4: HSP70. Right blot probed with DnaK mAb (Prod. No. ADI-SPA-880) - Lane 5: MW Marker, Lane 6: DnaK (Prod. No. ADI-SPP-630), Lane 7: HSP70.
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ADI-ESP-555 SDS-PAGE ADI-ESP-555 WB

Product Literature References

Interaction of heat shock protein 70 with membranes depends on the lipid environment: G. Armijo, et al.; Cell Stress Chaperones (2014), Abstract;
Endotoxin contamination in recombinant human heat shock protein 70 (Hsp70) preparation is responsible for the induction of tumor necrosis factor alpha release by murine macrophages: M.F. Tsan, et al. ; J. Biol. Chem. 278, 174 (2003), Application(s): In Vitro Assay using human samples, Abstract;

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Heat Shock Proteins & the Cellular Stress Response Catalog
Heat Shock Proteins & the Cellular Stress Response Catalog 09-Mar-10
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