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HO-1 (rat), ELISA kit

Highly sensitive colorimetric immunometric enzyme immunoassay kit with results in < 3 hours
 
ADI-EKS-810A 96 wells 635.00 USD
Do you need bulk/larger quantities?
 
  • Thoroughly validated in serum, plasma, cell lysates, and tissue extracts
  • Highly sensitive quantification of HO-1 surpass semi-quantitative Western blot results
  • Accurate and reproducible results in less than 3 hours
The HO-1 (rat), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.
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Product Specification

Alternative Name:Heme oxygenase 1
 
Sensitivity:0.036 ng/ml (range 0.195 - 12.5 ng/ml)
 
Assay Time:<3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of rat HO-1 in cell lysates, plasma, serum, and tissue. Cited sample type includes bronchoalveolar lavage fluid.
 
Species reactivity:Rat
 
Use/Stability:Store all components at +4°, except standard at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter plate, 5X Extraction reagent 2, Standard, Sample diluent, Wash buffer concentrate, Antibody, Conjugate, TMB Substrate, Stop solution 2
 
Scientific Background:Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.
 
UniProt ID:P06762
 
GenBank ID:J02722
 

Product Literature References

Can heat shock protein 32 be used for the early diagnosis of acute mesenteric ischemia?: S. Berhuni, et al.; Ulus. Cerrahi. Derg. 32, 1 (2016), Application(s): Quantified heme oxygenase (HO) -1 concentrations, Abstract; Full Text
Effects of heme oxygenase-1 in the intestine on the intestinal barrier function of rats with hemorrhagic shock: X.Y. Gao, et al.; Int. J. Clin. Exp. Med. 9, 17367 (2016), Application(s): Measurement of HO-1 in rat intestine, Abstract; Full Text
Evaluation of Pulmonary Toxicity of Zinc Oxide Nanoparticles Following Inhalation and Intratracheal Instillation: Y. Morimoto, et al.; Int. J. Mol. Sci. 17, 1241 (2016), Application(s): Concentrations of rat HO-1 in BALF supernatant measurement, Abstract; Full Text
The DPP4 Inhibitor Linagliptin Protects from Experimental Diabetic Retinopathy: N. Dietrich, et al.; PLoS One 11, e0167853 (2016), Abstract;
FGF9-induced changes in cellular redox status and HO-1 upregulation are FGFR-dependent and proceed through both ERK and AKT to induce CREB and Nrf2 activation: J.I. Chuang, et al.; Free Radic. Biol. Med. 89, 274 (2015), Application(s): Western Blot, Abstract;
Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos Pathway: F. Han, et al.; Cell. Physiol. Biochem. 36, 1480 (2015), Application(s): ELISA using rat aortic homogenates , Abstract; Full Text
Recovery of redox homeostasis altered by CuNPs in H4IIE liver cells does not reduce the cytotoxic effects of these NPs: An investigation using aryl hydrocarbon receptor (AhR) dependent antioxidant activity: M. Connolly, et al.; Chem. Biol. Interact. 228, 57 (2015), Abstract;
Pulmonary toxicity of printer toner following inhalation and intratracheal instillation: Y. Morimoto, et al.; Inhal. Toxicol. 25, 679 (2013), Application(s): ELISA using rat bronchoalveolar lavage fluid, Abstract;
Upregulation of cytoprotective defense mechanisms and hypoxia-responsive proteins imparts tolerance to acute hypobaric hypoxia: K. Jain, et al.; High Alt. Med. Biol. 14, 65 (2013), Application(s): ELISA using rat heart tissue homogenate, Abstract;
Pulmonary inflammation of well-dispersed multi-wall carbon nanotubes following intratracheal instillation: M. Horie, et al; Materials 5, 2833 (2012), Application(s): ELISA using rat bronchoalveolar lavage fluid, Full Text
Ischemic preconditioning and remote ischemic preconditioning have protective effect against cold ischemia–reperfusion injury of rat small intestine: I. Saeki, et al.; Pediatr. Surg. Int. 27, 857 (2011), Application(s): ELISA using rat serum, Abstract;
4-Methylcatechol-induced heme oxygenase-1 exerts a protective effect against oxidative stress in cultured neural stem/progenitor cells via PI3 kinase/Akt pathway: Y. Furukawa, et al.; Biomed. Res. 31, 45 (2010), Application(s): ELISA using rat cell extracts, Abstract; Full Text
Up-regulation of heme oxygenase-1 in rat spleen after aniline exposure: J. Wang, et al.; Free Radic. Biol. Med. 48, 513 (2010), Application(s): ELISA using rat spleen tissue lysates, Abstract; Full Text

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