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HO-1 (human), ELISA kit

Highly sensitive HO-1 ELISA kit enabling detection of HO-1 in cell lysates and tissue extracts in < 2.5 hours.
 
ADI-EKS-800 96 wells 635.00 USD
Do you need bulk/larger quantities?
 
  • Highly sensitive assay, measure as little as 0.78 ng/ml of HO-1
  • Reliable and reproducible results day-after-day and lot-after-lot
  • Higher throughput format, assay up to 40 samples in duplicate in just 2.5 hours
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
The HO-1 (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 2.5 hours. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.
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Product Specification

Alternative Name:Heme oxygenase 1
 
Sensitivity:0.78 ng/ml (range 0.78 - 25 ng/ml)
 
Assay Time:<2.5 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of human heme oxygenase-1 in cell lysates and tissue extracts. Cited sample types include serum and plasma.
 
Species reactivity:Human
 
Crossreactivity:Human HO-1 (100%). No reactivity with HO-2 or HO-3.
 
Use/Stability:Store all components at +4°, except standard at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter plate, 5X Extraction reagent, Standard, Sample diluent, Wash buffer concentrate, Antibody, Antibody diluent, HRP Conjugate, Conjugate diluent, TMB Substrate, Stop solution 2
 
Scientific Background:Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.
 
UniProt ID:P09601
 

Product Literature References

Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis: U. Wasik, et al.; Sci. Rep. 7, 44769 (2017), Abstract; Full Text
Cellular Effects in an In Vitro Human 3D Cellular Airway Model and A549/BEAS-2B In Vitro Cell Cultures Following Air Exposure to Cerium Oxide Particles at an Air-Liquid Interface: I.M. Kooter, et al.; Applied in vitro Toxicology 2, 56 (2016), Application(s): Measurement of heme oxygenase (HO-1) protein expression in human cells, Abstract;
Erythropoietin Levels Increase during Cerebral Malaria and Correlate with Heme, Interleukin-10 and Tumor Necrosis Factor-Alpha in India: E. Dalko, et al.; PLoS One 11, e0158420 (2016), Application(s): Determination of EPO, total heme, hemopexin and heme oxygenase (HO)-1 levels in plasma, Abstract; Full Text
Heme oxygenase-1 regulates inflammation and mycobacterial survival in human macrophages during Mycobacterium tuberculosis infection: C.R. Scharn, et al.; J. Immunol. 196, 4641 (2016), Abstract;
Astrocyte overexpression of heme oxygenase-1 improves outcome after intracerebral hemorrhage: J. Chen-Roetling, et al.; Stroke 46, 1093 (2015), Application(s): HO-1 levels in injected and contralateral striata, Abstract; Full Text
Differential expression of heat shock proteins in fish hepatocytes under hypoxic condition: E. Padmini, et al.; Int. J. Fish. Aquat. Stud. 3, 447 (2015), Application(s): Quantification of HO-1, Full Text
Relationship between NOX4 level and angiotensin II signaling in Gitelman’s syndrome. Implications with hypertension: L.A. Calò, et al.; Int. J. Clin. Exp. Med. 8, 7487 (2015), Application(s): ELISA using human cell lysates, Abstract; Full Text
Increased microRNA-146a levels in plasma of patients with newly diagnosed type 2 diabetes mellitus: Y. Rong, et al.; PLoS One 8, e73272 (2013), Application(s): HO-1 levels in human plasma, Abstract; Full Text
Transcription factor Nrf2 protects renal dopamine D1 receptor function during oxidative stress: A.A. Banday, et al.; Hypertension 62, 512 (2013), Application(s): HO-1 levels in renal proximal tubules, Abstract; Full Text
Antioxidant and cytoprotective effects of L-Serine on human endothelial cells: M.N. Maralani, et al.; Res. Pharm. Sci. 7, 209 (2012), Application(s): ELISA using HUVEC cell lysates, Abstract; Full Text
Heme oxygenase microsatellite polymorphism, oxidative stress, glycemic control, and complication development in type 2 diabetes patients: S.W. Choi, et al.; Free Radic. Biol. Med. 53, 60 (2012), Application(s): HO-1 levels in human plasma, Abstract;
Plasma heme oxygenase-1 concentration in relation to impaired glucose regulation in a non-diabetic Chinese population.: W. Bao, et al.; PLoS One 7, e32223 (2012), Abstract; Full Text
Schizophrenia-like features in transgenic mice overexpressing human HO-1 in the astrocytic compartment: W. Song, et al.; J. Neurosci. 8, 32 (2012), Application(s): ELISA using transgenic mouse brain, Abstract; Full Text
Unregulated brain iron deposition in transgenic mice over-expressing HMOX1 in the astrocytic compartment: W. Song, et al.; J. Neurochem. 123, 325 (2012), Application(s): ELISA using transgenic mouse brain, Abstract;
First in-human study demonstrating pharmacological activation of heme oxygenase-1 in human: A. Bharucha, et al.; Clin. Pharmacol. Ther. 87, 187 (2010), Application(s): EIA using human plasma, Abstract;
Increased serum HO-1 in hemophagocytic syndrome and adult-onset Still's disease: use in the differential diagnosis of hyperferritinemia: Y. Kirino, et al.; Arthritis Res. Ther. 7, R616 (2005), Application(s): EIA using human serum, Abstract;
Biliverdin reductase, a novel regulator for induction of activating transcription factor-2 and heme oxygenase-1: M.D. Maines, et al. ; J. Biol. Chem. 279, 19916 (2004), Application(s): EIA using human cell lysates, Abstract;
Hemoglobin scavenger receptor CD163 mediates interleukin-10 release and heme oxygenase-1 synthesis: anti inflammatory monocyte-macrophage responses in vitro, in resolving skin blisters in vivo, and after cardiopulmonary bypass surgery: R.C. Landis, et al. ; Circ. Res. 94, 119 (2004), Application(s): EIA using human cell lysates, Abstract;

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