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HSP70 high sensitivity ELISA kit

Only commercially available kit specifically validated and sensitive enough to quantify HSP70 (HSP72) in serum and plasma samples.
 
ADI-EKS-715 96 wells 567.00 USD
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  • Highly sensitive measurement, quantify as little as 90pg/ml Hsp70 (Hsp72)
  • Fully validated for use with serum and plasma samples
  • High specificity, measures stress-induced Hsp70 (Hsp72) with negligible reactivity from other Hsp70 family members (e.g. Hsc70/Hsp73, Grp78)
  • Higher throughput  format with results in just 4.5 hours for up to ~40 samples in duplicate
The HSP70 high sensitivity EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 4.5 hours. Absorbance is read at 450 nm. This is the only commercially available kit specifically validated and sensitive enough to quantify HSP70 (HSP72) in serum and plasma samples, as referenced by the Cell Stress Society International.

Product Specification

Alternative Name:Heat shock protein 70, HSP72
 
Sensitivity:90 pg/ml (range 0.20-12.5 ng/ml)
 
Assay Time:4.5 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of HSP70 in serum and plasma of human, mouse, and rat origin. Cited sample(s) include cell lysates and urine.
 
Species reactivity:Human, Mouse, Rat
 
Crossreactivity:HSP72 (human) (100%), HSP70 (rat) (117.6%), HSP70 (salmon) (0.8%), HSP70B’ (human) (5.4%), DnaK (E.coli) (0.5%), and <0.016%: Hsc70 (bovine), Grp78 (hamster), HSP71 (M. tuberculosis)
 
Use/Stability:Store all components at +4°, except standard at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter plate, Assay buffer 28, Standard, Wash buffer concentrate, Antibody, Conjugate, TMB Substrate, Stop solution 2
 
Scientific Background:The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
 
UniProt ID:P0DMV8 (HSPA1A), P0DMV9 (HSPA1B)
 

Product Literature References

In vitro and in vivo antivirus activity of an anti-programmed death-ligand 1 (PD-L1) rat-bovine chimeric antibody against bovine leukemia virus infection: A. Nishimori, et al.; PLoS One 12, e0174916 (2017), Application(s): Selection of PD-L1/EGFP on Calf Peripheral Blood Mononuclear Cells (PMBCs) , Abstract; Full Text
Treatment with Geranylgeranylacetone Induces Heat Shock Protein 70 and Attenuates Neonatal Hyperoxic Lung Injury in a Model of Bronchopulmonary Dysplasia: S. Tokuriki, et al.; Lung (2017), Application(s): HSP70 ELISA on Human Neonatal Serum, Abstract; Full Text
Evaluation of tympanic temperature and thermal sensation responses during exercise to verify the positive effects of wearing germanium-coated functional clothing: S.Y. Cho, et al.; J. Phys. Ther. Sci. 28, 1860 (2016), Application(s): ELISA, human blood and serum levels, Abstract; Full Text
Functional effects of milk bioactive peptides on skeletal muscle of rats: C.S. Moura, at al.; Food Res. Int. 84, 18 (2016), Application(s): Measurement of serum HSP70 level,
Heat shock protein 70 and antibodies to heat shock protein 60 are associated with cerebrovascular atherosclerosis: R. Galovic, et al.; Clin. Biochem. 49, 66 (2016), Application(s): Measurements of serum concentrations of Hsp70, Abstract;
Influence of osmotic stress on thermal perception and thermoregulation in heat is different between sedentary and trained men: K. Tokizawa, et al.; Physiol. Behav. 161, 66 (2016), Application(s): HSP70 levels were measured in male serum samples, Abstract;
Salivary extracellular heat shock protein 70 (eHSP70) levels increase after 59 min of intense exercise and correlate with resting salivary secretory immunoglobulin A (SIgA) levels at rest: Y. Murase, et al.; Cell Stress Chaperones 21, 261 (2016), Abstract; Full Text
Soluble heat shock protein 70 members in patients undergoing allogeneic hematopoietic cell transplantation: N. Mirza, et al.; Transpl. Immunol. 36, 25 (2016), Application(s): Heat shock protein detection, Abstract;
Urinary Biomarkers Indicative of Apoptosis and Acute Kidney Injury in the Critically Ill: S.T. Vaara, et al.; PLoS One 11, e0149956 (2016), Application(s): Determination of plasma and urine HSP70, Abstract; Full Text
A comparison of two commercially available ELISA methods for the quantification of human plasma heat shock protein 70 during rest and exercise stress: B.J. Lee, et al.; Cell Stress Chaperones 20, 917 (2015), Application(s): ELISA using human plasma, Abstract; Full Text
Analysis of serum heat shock protein 70 (HSPA1A) concentrations for diagnosis and disease activity monitoring in patients with rheumatoid arthritis: S.R. Najafizageh, et al.; Cell Stress Chaperones 20, 537 (2015), Application(s): Immunosorbent assay, Abstract; Full Text
COPD and levels of Hsp70 (HSPA1A) and Hsp27 (HSPB1) in plasma and lymphocytes among coal workers: a case-control study: X. Cui, et al.; Cell Stress Chaperones 20, 473 (2015), Application(s): EIA using human plasma, Abstract;
Early inflammatory response in polytraumatized patients: Cytokines and heat shock proteins. A pilot study: M.C. Guisasola, et al.; Orthop. Traumatol. Surg. Res. 101, 607 (2015), Application(s): ELISA using human serum, Abstract;
Effects of cytarabine on activation of human T cells - cytarabine has concentration-dependent effects that are modulated both by valproic acid and all-trans retinoic acid: E. Ersvaer, et al.; BMC Pharmacol. Toxicol. 16, 12 (2015), Application(s): ELISA using human supernatant, Abstract; Full Text
Effects of different fluid replacements on serum HSP70 and lymphocyte DNA damage in college athletes during exercise at high ambient temperatures: H.T. Roh, et al.; J. Sport Health Sci. (2015), Application(s): ELISA using human serum,
Human monocyte heat shock protein 72 responses to acute hypoxic exercise after 3 days of exercise heat acclimation: B.J. Lee, et al.; Biomed. Res. Int. 2015, 849809 (2015), Application(s): ELISA using human plasma, Abstract; Full Text
Influence of sex on cytokines, heat shock protein and oxidative stress markers in response to an acute total body resistance exercise protocol: R. Benini, et al.; J. Exercise Science and Fitness (2015), Application(s): ELISA, Full Text
Release of endogenous Hsp72 on the survival of sepsis in rats: T.N. Tsai, et al.; J. Surg. Res. 198, 165 (2015), Application(s): ELISA, Abstract;
Adrenergic and glucocorticoid modulation of the sterile inflammatory response: S. Cox, et al.; Brain Behav. Immun. 36, 183 (2014), Application(s): EIA using human plasma samples, Abstract;
Evaluation of Rhodiola rosea supplementation on skeletal muscle damage and inflammation in runners following a competitive marathon: R. Shanely, et al.; Brain Behav. Immun. 39, 204 (2014), Application(s): EIA using human plasma samples, Abstract;
Presence of heat shock protein 70 in secondary lymphoid tissue correlates with stroke prognosis: M. Gómez-Choco, et al.; J. Neuroimmunol. 270, 67 (2014), Application(s): EIA using human plasma from stroke victims, Abstract;
Urinary neutrophil gelatinase-associated lipocalin predicts graft loss after acute kidney injury in kidney transplant: J. Ramírez-Sandoval, et al.; Biomarkers 19, 63 (2014), Application(s): Analysis of uHsp72 in kidney transplant recipients, Abstract;
Induction of Heat Shock Protein 70 Ameliorates Ultraviolet-Induced Photokeratitis in Mice: A. Lennikov, et al.; Int. J. Mol. Sci. 14, 2175 (2013), Application(s): EIA using mouse cornea cell extracts., Abstract;
Response of circulating heat shock protein 70 and anti-heat shock protein 70 antibodies to catheter ablation of atrial fibrillation: J. Kornej, et al.; J. Transl. Med. 11, 49 (2013), Application(s): EIA using human plasma samples, Abstract; Full Text
Electrophysiological correlation and prognostic impact of heat shock protein 27 in atrial fibrillation: Y.F. Hu, et al.; Circ. Arrhythm. Electrophysiol. 5, 334 (2012), Application(s): ELISA using human serum, Abstract; Full Text
Plasma heat shock protein 72 as a biomarker of sarcopenia in elderly people: K. Ogawa, et al.; Cell Stress Chaperones 17, 349 (2012), Application(s): EIA using human plasma samples, Abstract; Full Text
Recovery from ischemic acute kidney injury by spironolactone administration: K. Sánchez-Pozos, et al.; Nephrol. Dial. Transplant. 27, 3160 (2012), Application(s): Urinary Hsp72 protein levels, Abstract;
Distinguishing integral and receptor-bound heat shock protein 70 (Hsp70) on the cell surface by Hsp70-specific antibodies: G. Multhoff & L. Hightower; Cell Stress Chaperones 16, 251 (2011), Application(s): EIA of extracellular HSP in serum and plasma samples, Abstract; Full Text
Serum heat shock protein 70 and oxidized LDL in patients with type 2 diabetes: does sex matter?: M. Nakhjavani, et al.; Cell Stress Chaperones 16, 195 (2011), Application(s): EIA using human serum, Abstract; Full Text
Plasma levels of Hsp70 and anti-Hsp70 antibody predict risk of acute coronary syndrome: X. Zhang, et al.; Cell Stress Chaperones 15, 675 (2010), Application(s): EIA using human plasma, Abstract;
No endogenous circadian rhythm in resting plasma Hsp72 concentration in humans: M.B. Fortes MB & M. Whitham; Cell Stress Chaperones 14, 273 (2009), Application(s): EIA using human plasma, Abstract;

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