HSP70 high sensitivity ELISA kit

Only commercially available kit specifically validated and sensitive enough to quantify HSP70 (HSP72) in serum and plasma samples.



ADI-EKS-715	96 wells	625.00 USD

Sensitive - quantify as little as 90pg/ml Hsp70 (Hsp72)
Reliable - fully validated for use with serum and plasma samples
Specific - measure stress-induced Hsp70 (Hsp72) with negligible reactivity from other Hsp70 family members (e.g. Hsc70/Hsp73, Grp78)
Higher throughput - measure ~40 samples in duplicate in just 4.5 hours

The HSP70 high sensitivity EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 4.5 hours. Absorbance is read at 450 nm. This is the only commercially available kit specifically validated and sensitive enough to quantify HSP70 (HSP72) in serum and plasma samples, as referenced by the Cell Stress Society International.

Product Specification

Alternative Name:	Heat shock protein 70, HSP72

Sensitivity:	90 pg/ml (range 0.20-12.5 ng/ml)

Kit/Set Contains:	Microtiter plate, Assay buffer 28, Standard, Wash buffer concentrate, Antibody, Conjugate, TMB Substrate, Stop solution 2

Application:	For the quantitative determination of HSP70 in serum and plasma of human, mouse, and rat origin.

Use/Stability:	Store all components at +4°, except standard at -20°.

Miscellaneous/General:	The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Product Literature References

Plasma levels of Hsp70 and anti-Hsp70 antibody predict risk of acute coronary syndrome.: X. Zhang, et al.; Cell Stress Chaperones 15, 675 (2010), Application(s): EIA using human plasma, Abstract;

Serum heat shock protein 70 and oxidized LDL in patients with type 2 diabetes: does sex matter?: M. Nakhjavani, et al.; Cell Stress Chaperones (2010), Application(s): EIA using human serum, Abstract;

No endogenous circadian rhythm in resting plasma Hsp72 concentration in humans.: M. B. Fortes MB, M. Whitham; Cell Stress Chaperones 14, 273 (2009), Application(s): EIA using human plasma, Abstract;