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HSP70 ELISA kit

Widely used ELISA HSP70 kit for chaperone and stress research.
 
ADI-EKS-700B 96 wells 562.00 USD
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  • Sensitive measurement of HSP70, measuring as low as 200 pg/ml
  • Fully quantitative results compared to Western blot analysis
  • Highly specific, with minimal cross-reactivity with other HSP
  • Validated in stressed and non-stressed samples
The HSP70 EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 4.5 hours. Absorbance is read at 450 nm. This kit is ideal for measuring intracellular HSP70 (HSP72). Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.

Product Specification

Alternative Name:Heat shock protein 70, HSP72
 
Sensitivity:200 pg/ml (range 780 - 50,000 pg/ml)
 
Assay Time:4.5 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of HSP70 in cell lysates and tissue of human, mouse, and rat origin. Prod. No. ADI-EKS-715 is recommended for the detection of Hsp70 in serum and plasma samples.
 
Species reactivity:Human, Mouse, Rat
 
Use/Stability:Store all components at +4°, except standard at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter plate, 5X Extraction reagent, Standard, Sample diluent 2, Wash buffer concentrate, Antibody, Conjugate, TMB Substrate, Stop solution 2
 
Scientific Background:The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
 
UniProt ID:P0DMV8 (HSPA1A), P0DMV9 (HSPA1B)
 

Product Literature References

Secretion of cytokines and heat shock protein (HspA1A) by ovarian cancer cells depending on the tumor type and stage of disease: M. Nowak, et al.; Cytokine 89, 136 (2017), Application(s): HSP70 in ovarian cancer cells, Abstract;
Diagnostic Role for Antibodies against Heat Sock Protein 70 In Perilymphatic Fluid of Children with Cochlear Implant Surgery: A Case Control Study in Tehran, Iran: M.R. Shokrollahi, et al.; Int. J. Curr. Res. Aca. Rev. 4, 184 (2016), Application(s): HSP70 levels measured in blood samples , Full Text
Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping: N. Frara, et al.; BMC Musculoskelet. Dis. 17, 34 (2016), Application(s): HSP72 measured in tissue lysates, Abstract; Full Text
Myokines In Response To A Tournament Season Among Young Tennis Players: K. Witek, et al.; Biomed Res. Int. 2016, Article ID 1460892 (2016), Application(s): Blood sampling and cytokine analysis, serum HSP analysis, Abstract; Full Text
Physiological, Morphological and Behavioural Responses of Self-Feeding Precocial Chicks Copying with Contrasting Levels of Water Salinity during Development: A.R. Rocha, et al.; PLoS One 11, e0165364 (2016), Application(s): Hsp70 concentration fromthe erythrocyte lysates , Abstract; Full Text
Antioxidant Reviving Role of Mint-Tea on Placenta During Gestational Diabetes Mellitus: P. Ekambaram, et al.; International Journal of Science and Research 4, 501 (2015), Application(s): ELISA using human GDM (Gestational diabetes mellitus) placental tissue homogenate, Full Text
Could the Anti-Chaperone VER155008 Replace Temozolomide for Glioma Treatment: L. Shervington, et al.; J. Cancer 6, 786 (2015), Application(s): ELISA using human cell lysate, Abstract; Full Text
Effect of copper nanoparticles and copper sulphate on oxidation stress, cell apoptosis and immune responses in the intestines of juvenile Epinephelus coioides: W. Tao, et al.; Fish Shellfish Immunol. 44, 674 (2015), Application(s): ELISA, Abstract;
Effect Of Early Heat Acclimation On Immunity And Some Intestinal Functions And Heat Shock Protein Post-Weaned Rabbits During Summer Season: A.S. O EI-Badry, et al.; Egypt. Poult. Sci. 35, 123 (2015), Application(s): ELISA using rabbit small intestine cell supernatant, Full Text
Increased plasma levels of heat shock protein 70 associated with subsequent clinical conversion to mild cognitive impairment in cognitively healthy elderly: S.J. Son, et al.; PLoS One 10, e0119180 (2015), Application(s): ELISA using human plasma, Abstract; Full Text
Lazaroids U83836E and U74389G are potent, time-dependent inhibitors of caspase-1: M. Kawarski, et al.; Chem. Biol. Drug Des. 86, 1049 (2015), Application(s): Analysis of tissue lysates, Abstract; Full Text
Role of Hsp70 and the Associated Signaling Molecules in Fish Adipocytes during Pollutants Induced Hypoxia: P. Ekambaram, et al.; IOSR J. Environ. Sci. Toxicol. Food Technol. 9, 16 (2015), Application(s): ELISA using fish adipocytes, Full Text
Adrenergic and glucocorticoid modulation of the sterile inflammatory response: S. Cox, et al.; Brain Behav. Immun. 36, 183 (2014), Application(s): EIA using human plasma samples, Abstract;
Basal and stress-induced Hsp70 are modulated by ataxin-3: C.P. Reina, et al.; Cell Stress Chaperones 17, 729 (2012), Application(s): EIA using mouse tissue extract, Abstract; Full Text
Impact of seasonal variation on HSP70 expression quantitated in stressed fish hepatocytes: E. Padmini, et al. ; Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 151, 278 (2008), Application(s): EIA using fish cell lysates, Abstract;
The effect of the rate of heat storage on serum heat shock protein 72 in humans: F. Amorim, et al. ; Eur. J. Appl. Physiol. 104, 965 (2008), Application(s): EIA using human serum, Abstract;
Maximal eccentric exercise induces a rapid accumulation of small heat shock proteins on myofibrils and a delayed HSP70 response in humans: G. Paulsen, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 293, R844 (2007), Application(s): EIA using human vastus lateralis muscle tissue, Abstract; Full Text

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