Anti-HSP60 IgG/A/M (human), ELISA kit

Most comprehensive portfolio of heat shock protein (chaperone) products commercially available.



ADI-EKS-650	96 wells	590.00 USD

Quantitative - fully quantitate levels of auto-antibodies to HSP60
Higher throughput - obtain results from up to 41 samples in duplicate in less than 4 hours
Easy-to-use - liquid color-coded reagents and pre-coated plates reduce errors and save time in the lab

This assay allows for reproducible, accurate and precise determination of IgG , IgA and IgM antibodies (total) to human Hsp60 in serum. The ELISA kit uses recombinant human Hsp60 bound to the wells of the plate to bind anti-human Hsp60 antibodies present in human serum. The captured anti-human Hsp60 antibodies are detected with a HRP conjugated goat polyclonal antibody specific for human IgG, IgA and IgM molecules. The assay is developed with tetramethylbenzidine substrate pro ducing a blue color in proportion to the amount of captured anti -human Hsp60 antibodies.

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Product Specification

Sensitivity:	2.88 ng/ml (range 7.81 - 250 ng/ml)

Kit/Set Contains:	Microtiter plate, Standard, Sample diluent 1, Wash buffer concentrate, HRP Conjugate, TMB Substrate, Stop solution 2

Application:	For the quantitative determination of anti-HSP60 IgG/A/M autoantibodies in human serum.

Long Term Storage:	+4°C

Miscellaneous/General:	Hsp60 is a member of the chaperonin family of heat shock proteins, with homologs functioning in the cytosol and mitochondria to fold nascent and aggregated proteins. Hsp60 is the eukaryotic homolog of the E. coli GroEL protein, and forms a multimeric complex in the mitochondria with Hsp10 (Cpn10) to form a large central cavity in which ATP-dependent protein folding takes place. TRiC/CCT, a eukaryotic relative of Hsp60, is expressed in the cytosol and participates in the folding of actin and tubulin substrates, but lacks any association with an Hsp10-like co-factor.